beta Actin 抗体 826 文献

Mouse Monoclonal| カタログ番号: 60008-1-Ig |クローン番号: 7D2C10

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20,000円

(希望販売価格)

容量: 150 μg/150 μl

Choose quantity:

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distributor

種特異性:
human, mouse, rat, pig, yeast, plant, Zebrafish, A. sphaerocephala Krasch, bovine, C. tubulosa, cat, chicken, Drosophila, duck, eels, Eelworm, Gekko japonicus, Hamster, orange-spotted grouper, ostrich, rabbit, Rare minnows, rice, sheep, swine

WB ポジティブ:
multi-cells/tissue, A549 cells, HEK-293 cells, MCF-7 cells, arabidopsis whole plant tissue, whole Nematode tissue, HeLa cells, whole yeast cells, HeLa cells

IHC ポジティブ:
human colon tissue, human brain tissue, human kidney tissue, human lung tissue, human spleen tissue, human ovary tissue, human testis tissue, human placenta tissue, human heart tissue

IF ポジティブ:
HepG2 cells

FC ポジティブ:
HepG2 cells

推奨希釈倍率:
WB : 1:5000-1:50000
IHC : 1:50-1:500
IF : 1:20-1:200

製品情報


由来:
Mouse

精製方法:
Caprylic acid/ammonium sulfate precipitation

アイソタイプ:
IgM

バッファー/保存方法:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC.

ターゲット情報


正式名:
actin, beta

計算分子量:
375aa,42 kDa

検出分子量:
42 kDa

GenBank accession number:

Gene ID (NCBI):

遺伝子シンボル:
ACTB

別名:
ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, F actin, β actin
背景説明

Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 37-40 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody can recognize all the actins. The isotype of this antibody is mouse IgM. It can be detected with the secondary antiody "SA00001-1" Peroxidase-conjugated Affinipure Goat Anti-Mouse IgG(H+L). (9173887, 11217076)


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