IFT43 Polyclonal antibody

IFT43 Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, canine and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 24338-1-AP

Synonyms

C14orf179, IFT43



Tested Applications

Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMDCK cells, hTERT-RPE1 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

24338-1-AP targets IFT43 in WB, IHC, IF, ELISA applications and shows reactivity with human, canine samples.

Tested Reactivity human, canine
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IFT43 fusion protein Ag19434 相同性解析による交差性が予測される生物種
Full Name chromosome 14 open reading frame 179
Calculated molecular weight 213 aa, 24 kDa
GenBank accession numberBC010436
Gene symbol C14orf179
Gene ID (NCBI) 112752
RRIDAB_2749824
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IFT43, encoded by C14ORF179, is a subunit of the intraflagellar transport complex A (IFT-A) of primary cilia. The IFT-A is implicated in retrograde ciliary transport along axonemal microtubules. Defects in subunits of IFT-A has been associated with skeletal ciliopathies, including Sensenbrenner syndrome, Jeune syndrome, and Ellis-van-Creveld syndrome. Mutations in C14ORF179 has been linked to Sensenbrenner syndrome. (21378380)

Protocols

Product Specific Protocols
IHC protocol for IFT43 antibody 24338-1-APDownload protocol
IF protocol for IFT43 antibody 24338-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Brain

De novo purine biosynthesis is a major driver of chemoresistance in glioblastoma.

Authors - Jack M Shireman
mouseIF

Proc Natl Acad Sci U S A

Primary cilia signaling mediates intraocular pressure sensation.

Authors - Na Luo
humanIF

Elife

Dynein-2 intermediate chains play crucial but distinct roles in primary cilia formation and function.

Authors - Laura Vuolo
mouse

Hum Mol Genet

Ift172 conditional knock-out mice exhibit rapid retinal degeneration and protein trafficking defects.

Authors - Priya R Gupta
mouseWB

J Cell Physiol

Ttc39c is upregulated during skeletal muscle atrophy and modulates ERK1/2 MAP kinase and hedgehog signaling.

Authors - Caleb S Hayes