FBXO5 Polyclonal antibody

FBXO5 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10872-1-AP

Synonyms

Early mitotic inhibitor 1, EMI1, F box only protein 5, F box protein 5, FBX5, FBXO5



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, human ovary tissue, K-562 cells, mouse kidney tissue, rat liver tissue
Positive IHC detected inhuman placenta tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10872-1-AP targets FBXO5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FBXO5 fusion protein Ag1296 相同性解析による交差性が予測される生物種
Full Name F-box protein 5
Calculated molecular weight 54 kDa
Observed molecular weight 56 kDa, 45 kDa
GenBank accession numberBC018905
Gene symbol FBXO5
Gene ID (NCBI) 26271
RRIDAB_2247034
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FBXO5, also named as EMI1 and FBX5, is a key regulator of the normal cell cycle. It is a a zinc finger protein expressed by a variety of adult tissues and especially in proliferating Ki-67-positive cells. It binds to the APC activators CDC20 and FZR1/CDH1 to prevent APC activation. FBXO5 can also bind directly to the APC to inhibit substrate-binding.

Protocols

Product Specific Protocols
WB protocol for FBXO5 antibody 10872-1-APDownload protocol
IHC protocol for FBXO5 antibody 10872-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

CRL4AMBRA1 is a master regulator of D-type cyclins.

Authors - Daniele Simoneschi
humanWB

Nat Commun

DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation

Authors - Xiaohua Xu
humanWB

J Cell Mol Med

An upstream open reading frame regulates vasculogenic mimicry of glioma via ZNRD1-AS1/miR-499a-5p/ELF1/EMI1 pathway.

Authors - Mo Wang
humanWB

J Cancer Res Clin Oncol

Identification of aberrantly expressed F-box proteins in squamous-cell lung carcinoma.

Authors - Kai Wang
  • KD Validated
humanWB

Exp Cell Res

The transcript ENST00000444125 of lncRNA LINC01503 promotes cancer stem cell properties of glioblastoma cells via reducing FBXW1 mediated GLI2 degradation.

Authors - Pan Wei
humanWB

Mol Cell Probes

Exploring the role of FBXO5 in gastric cancer

Authors - Junchang Zhang