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FBXW7 Polyclonal antibody
FBXW7 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 55290-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, HEK-293 cells, MCF-7 cells, human brain tissue |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human skin cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 10 publications below |
Product Information
55290-1-AP targets FBXW7 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, rat, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | F-box and WD repeat domain containing 7 |
| Calculated molecular weight | 80 kDa |
| Observed molecular weight | 100-110 kDa, 66-75 kDa |
| GenBank accession number | NM_018315 |
| Gene symbol | FBXW7 |
| Gene ID (NCBI) | 55294 |
| RRID | AB_2881300 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FBXW7, also named as FBW7, FBX30, SEL10 and hAgo, is a substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. It probably recognizes and binds to phosphorylated target proteins. FBXW7 is involved in the degradation of cyclin-E, MYC, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. FBXW7 is a general tumor suppressor in human cancer (PMID: 17909001). FBXW7 has 3 isoforms (α/β/γ) with the calculated molecular mass of 80 kDa, 70 kDa and 66 kDa, and apparent molecular mass of 100-110 kDa and 66-75 kDa (PMID: 18559665). This antibody is specific to FBXW7 and it can recognize all the isoforms of FBXW7.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FBXW7 antibody 55290-1-AP | Download protocol |
| IHC protocol for FBXW7 antibody 55290-1-AP | Download protocol |
| IF protocol for FBXW7 antibody 55290-1-AP | Download protocol |
| IP protocol for FBXW7 antibody 55290-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Cyclin E-induced replicative stress drives p53-dependent whole-genome duplication
| ||
iScience Polycomb repressive complex 2 binds and stabilizes NANOG to suppress differentiation-related genes to promote self-renewal | ||
Endocrinology Rictor regulates spermatogenesis by controlling Sertoli cell cytoskeletal organization and cell polarity in the mouse testis. | ||
Int J Biochem Cell Biol The deubiquitinase USP38 promotes cell proliferation through stabilizing c-Myc. | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Iram (Verified Customer) (09-17-2020) | FBX W7 is an excellent antibody for WB and IHC. Giving a very clear bands and sharp staining in tissue sections.
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