Human HSPD1 ELISA Kit

Sensitivity

7 pg/mL

Range

31.25-2000 pg/mL

Reactivity

Human

Cat no : KE00032

Synonyms

60 kDa chaperonin, Chaperonin 60, CPN60, GROEL, Heat shock protein 60, HLD4, HSP 60, HSP60, HSP65, HSPD1, HuCHA60, P60 lymphocyte protein, SPG13



Product Information

KE00032 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The HSPD1 ELISA kit is to be used to detect and quantify protein levels of endogenous HSPD1. The assay recognizes human HSPD1. A polyclonal antibody specific for HSPD1 has been pre-coated onto the microwells. The HSPD1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for HSPD1 is added to detect the captured HSPD1 protein. For signal development, horseradish peroxidase (HRP)-conjugated Anti-mouse antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

Product name Human HSPD1 ELISA Kit
Tests 1 X 96 well plate
Sample type Plasma, Serum
Assay type Sandwich
Sensitivity 7 pg/mL
Range 31.25-2000 pg/mL
Reactivity Human
Tested applications Sandwich ELISA
Gene ID (NCBI) 3329

Recovery

Sample TypeAverageRange
Plasma99%86%-119%

IntraAssay

Samplenmean ( pg/mL)SDCV%
120? ug/ml??%
220? ug/ml??%
320? ug/ml??%

InterAssay

Samplenmean ( pg/mL)SDCV%
120? ug/ml??%
220? ug/ml??%
320? ug/ml??%

Background Information

HSPD1(also known as HSP60) belongs to the chaperonin family and acts as a chaperone essential for protein folding. HSP60 is a widely expressed protein and has been connected with many aspects of cell functions including gene expression regulation, DNA replication, signal transduction, differentiation, apoptosis, and cellular senescence or immortalisation. Recently it has been reported that HSP60 was significantly up-regulated in squamous cell lung cancer.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms 60 kDa chaperonin, Chaperonin 60, CPN60, GROEL, Heat shock protein 60, HLD4, HSP 60, HSP60, HSP65, HSPD1, HuCHA60, P60 lymphocyte protein, SPG13

Publications

SpeciesSample TypeTitle
humancell culture supernatants

J Anal Methods Chem

Developing a Novel Indolocarbazole as Histone Deacetylases Inhibitor against Leukemia Cell Lines.

Authors - Wenjing Wang