Human INS ELISA Kit

Sensitivity

1.3 pmol/L

Range

7.8 - 500 pmol/L

Reactivity

Human

Cat no : KE00045

Synonyms

ILPR, INS, IRDN



Product Information

KE00045 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The INS ELISA kit is to be used to detect and quantify protein levels of endogenous INS. The assay recognizes human INS. An antibody specific for INS has been pre-coated onto the microwells. The INS protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for INS is added to detect the captured INS protein. For signal development, Sterptravidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Human INS ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma
Assay type Sandwich
Sensitivity 1.3 pmol/L
Range 7.8 - 500 pmol/L
Reactivity Human
Tested applications Sandwich ELISA
Gene ID (NCBI) 3630

Recovery

Sample TypeAverageRange
Human plasma100%82%-123%

IntraAssay

Samplenmean ( pmol/L)SDCV%
120295.727.79.4
220126.012.09.5
32064.66.29.6

InterAssay

Samplenmean ( pmol/L)SDCV%
124314.224.57.8
224149.213.28.8
32476.07.39.6

Background Information

Insulin is a peptide hormone, produced by beta cells of the pancreas, and is central to regulating carbohydrate and fat metabolism in the body. It participates in glucose utilization, protein synthesis and in the formation and storage of neutral lipids. Insulin is synthesized as a precursor molecule, proinsulin, which is processed prior to secretion. A- and B-peptides are joined together by a disulfide bond to form insulin, while the central portion of the precursor molecule is cleaved and released as the C-peptide. Defects in insulin results in type 1 diabetes mellitus.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms ILPR, INS, IRDN

Publications

SpeciesSample TypeTitle
humanserum

Front Cardiovasc Med

ANGPTL3 Is Involved in the Post-prandial Response in Triglyceride-Rich Lipoproteins and HDL Components in Patients With Coronary Artery Disease.

Authors - Xin Guo

Front Pharmacol

Lactobacillus reuteri strain 8008 attenuated the aggravation of depressive-like behavior induced by CUMS in high-fat diet-fed mice through regulating the gut microbiota

Authors - Canye Li