Human MMP-13 ELISA Kit

Sensitivity

1.2 pg/mL

Range

93.8-6000 pg/ml

Reactivity

Human

Cat no : KE00078

Synonyms

CLG3, Collagenase 3, Matrix metalloproteinase 13, MMP 13, MMP13, MMP-13



Product Information

KE00078 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The MMP-13 ELISA kit is to be used to detect and quantify protein levels of endogenous MMP-13. The assay recognizes human MMP-13. An antibody specific for MMP-13 has been pre-coated onto the microwells. The MMP-13 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for MMP-13 is added to detect the captured MMP-13 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Human MMP-13 ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants
Assay type Sandwich
Sensitivity 1.2 pg/mL
Range 93.8-6000 pg/ml
Reactivity Human
Tested applications Sandwich ELISA
Gene ID (NCBI) 4322

Recovery

Sample TypeAverageRange
Human plasma90%82%-100%
Cell culture supernatants86%79%-92%

IntraAssay

Samplenmean ( pg/mL)SDCV%
120928.669.57.5
2201,630.0101.76.2
3203,808.3227.16.0

InterAssay

Samplenmean ( pg/mL)SDCV%
1241,002.678.17.8
2241,833.0111.36.1
3244,422.9363.08.2

Background Information

MMP-13 (Matrix metalloproteinase 13), also known as collagenase-3, cleaves type I collagen and was previously thought to be the rodent analogue of human interstitial collagenase (MMP-1). MMP13 is considered to play a role in the tumor cell proliferation and invasion. MMP13 is a potential prognostic marker for colorectal cancer, breast cancer and other diseases (PMID: 20878116, PMID: 19787229).

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms CLG3, Collagenase 3, Matrix metalloproteinase 13, MMP 13, MMP13, MMP-13

Publications

SpeciesSample TypeTitle

Cancer Lett

PLD1 promotes tumor invasion by regulation of MMP-13 expression via NF-κB signaling in bladder cancer.

Authors - Yoshiyuki Nagumo
humanserum

J Clin Med

Zinc Administration and Improved Serum Markers of Hepatic Fibrosis in Patients with Autoimmune Hepatitis.

Authors - Kei Moriya
humancell culture supernatants

Int J Mol Sci

Exosomes Derived from Hypoxia-Cultured Human Adipose Stem Cells Alleviate Articular Chondrocyte Inflammaging and Post-Traumatic Osteoarthritis Progression

Authors - Ling-Hua Chang