Human SERPINA3 ELISA Kit
Cat no : KE00037
Synonyms
A1 Antichymotrypsin, AACT, ACT, Alpha 1 antichymotrypsin, GIG24, GIG25, Serpin A3, SERPINA3, SERPINA3,AACT
Validation Data Gallery
Product Information
KE00037 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The SERPINA3 ELISA kit is to be used to detect and quantify protein levels of endogenous SERPINA3. The assay recognizes human SERPINA3. An antibody specific for SERPINA3 has been pre-coated onto the microwells. The SERPINA3 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for SERPINA3 is added to detect the captured SERPINA3 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.
Product name | Human SERPINA3 ELISA Kit |
Tests | 1 X 96 well plate |
Sample type | Serum, Plasma |
Assay type | Sandwich |
Sensitivity | 0.55 ng/mL |
Range | 1-64 ng/mL |
Reactivity | Human |
Tested applications | Sandwich ELISA |
Gene ID (NCBI) | 12 |
Recovery
Sample Type | Average | Range |
---|---|---|
Human plasma | 99% | 82%-113% |
IntraAssay
Sample | n | mean ( ng/mL) | SD | CV% |
---|---|---|---|---|
1 | 20 | 28.9 | 1.6 | 5.5 |
2 | 20 | 5.6 | 0.4 | 6.9 |
3 | 20 | 1.2 | 0.1 | 9.7 |
InterAssay
Sample | n | mean ( ng/mL) | SD | CV% |
---|---|---|---|---|
1 | 24 | 26.8 | 1.9 | 6.9 |
2 | 24 | 5.3 | 0.3 | 6.1 |
3 | 24 | 1.4 | 0.1 | 8.6 |
Background Information
Human SerpinA3, also known as Alpha 1-antichymotrypsin (AACT), is a member of the serine protease inhibitor class, and is a plasma alpha globulin glycoprotein which increases in the blood during the inflammatory process. SerpinA3, is also an inhibitor of neutrophil cathepsin G, mast cell chymases and pancreatic chymotrypsin. SerpinA3 is produced primarily in the liver, and is identified as an acute-phase inflammatory protein. SerpinA3 deficiency has been associated with liver disease. SerpinA3 has also been implicated in the pathology of a number of devastating human diseases including chronic obstructive pulmonary disease (COPD), Parkinson's disease (PD), Alzheimer's disease (AD), Stroke, Cystic Fibrosis, Cerebral Haemorrhage and Multiple System Atrophy.
Properties
Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
Synonyms | A1 Antichymotrypsin, AACT, ACT, Alpha 1 antichymotrypsin, GIG24, GIG25, Serpin A3, SERPINA3, SERPINA3,AACT |
Publications
Species | Sample Type | Title |
---|---|---|
Dis Markers Identification of Protein Expression Changes in Hepatocellular Carcinoma through iTRAQ. |