IL-10 Polyclonal antibody

IL-10 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (5)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20850-1-AP

Synonyms

CSIF, IL 10, IL10, IL-10, IL10A, interleukin 10, TGIF



Tested Applications

Positive WB detected inRecombinant protein, ag14644

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20850-1-AP is IL-10 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human, rat, mouse, monkey, pig, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IL-10 fusion protein Ag14644 相同性解析による交差性が予測される生物種
Full Name interleukin 10
Calculated molecular weight 21 kDa
GenBank accession numberBC104252
Gene symbol IL10
Gene ID (NCBI) 3586
RRIDAB_2878752
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The human IL10 gene is 4.7 kb in size and is located on the long arm of chromosome 1. It contains five exons. Biologically functional IL-10 exists in the form of a 36 kDa homodimer composed of two non-covalently bonded mono-mers each with 160 amino acids length. IL-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL-10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL-10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.

Protocols

Product Specific Protocols
WB protocol for IL-10 antibody 20850-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nat Commun

B cells sustain inflammation and predict response to immune checkpoint blockade in human melanoma.

Authors - Johannes Griss
mouseIHC

Microbiome

Depletion of acetate-producing bacteria from the gut microbiota facilitates cognitive impairment through the gut-brain neural mechanism in diabetic mice.

Authors - Hong Zheng
ratIHC

Acta Biomater

A KPV-binding double-network hydrogel restores gut mucosal barrier in an inflamed colon.

Authors - Yingzheng Zhao
ratIHC

Acta Biomater

Bio-inspired Glycosylated Nano-hydroxyapatites Enhance Endogenous Bone Regeneration by Modulating Macrophage M2 Polarization

Authors - Dan Qiao
mouseIHC

Int J Biol Macromol

In situ polyphenol-adhesive hydrogel enhanced the noncarcinogenic repairing of KGF on the gut epithelial barrier on TNBS-induced colitis rats

Authors - Gaolong Lin
humanIHC

J Control Release

In situ hydrogel capturing nitric oxide microbubbles accelerates the healing of diabetic foot

Authors - Yingzheng Zhao