CD61 / Integrin Beta 3 Polyclonal antibody

CD61 / Integrin Beta 3 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 18309-1-AP

Synonyms

CD61, GP3A, GPIIIa, Integrin beta 3, Integrin beta-3, ITGB3



Tested Applications

Positive WB detected inHuman peripheral blood platelets, mouse spleen tissue, rat spleen tissue
Positive IP detected inmouse spleen tissue
Planning an IHC experiment? We recommend our IHCeasy ITGB3/CD61 Ready-To-Use IHC Kit. ITGB3/CD61 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Sample-dependent, check data in validation data gallery

Product Information

18309-1-AP targets CD61 / Integrin Beta 3 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat, mouse, Hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)
Calculated molecular weight 87 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC127667
Gene symbol ITGB3
Gene ID (NCBI) 3690
RRIDAB_2128759
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Integrin beta-3, also named as CD61 and GPIIIa, is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin beta-3 recognize the sequence R-G-D in a wide array of ligands and the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen which leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. Human integrin beta-3 has a calculated molecular weight of 87 kDa. As a result of glycosylation, the apparent molecular mass of integrin beta-3 is approximately 90-110 kDa.

Protocols

Product Specific Protocols
WB protocol for CD61 / Integrin Beta 3 antibody 18309-1-APDownload protocol
IP protocol for CD61 / Integrin Beta 3 antibody 18309-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Adv

Platelet-derived porous nanomotor for thrombus therapy.

Authors - Mimi Wan
WB

Nat Commun

Targeting Gα13-integrin interaction ameliorates systemic inflammation.

Authors - Ni Cheng
human,mouseIF

Biomaterials

Construction and evaluation of novel αvβ3 integrin ligand-conjugated ultrasmall star polymer micelles targeted glomerular podocytes through GFB permeation.

Authors - Chengyuan Huang
ratWB

Theranostics

A blood circulation-prolonging peptide anchored biomimetic phage-platelet hybrid nanoparticle system for prolonged blood circulation and optimized anti-bacterial performance.

Authors - Peipei Jin

Thromb Res

Repurposing of rilpivirine for preventing platelet β3 integrin-dependent thrombosis by targeting c-Src active autophosphorylation

Authors - Kui Liu
humanWB,IP

Elife

Binding blockade between TLN1 and integrin β1 represses triple-negative breast cancer.

Authors - Yixiao Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Sathyanarayani (Verified Customer) (04-05-2023)

I would highly recommend this primary antibody if you are looking for ITGB3. It produced really clear strong bands at 100kDa MW when used for western blot.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: mouse lysed blood
FH

Sathyanarayani (Verified Customer) (04-05-2023)

The ITGB3 antibody produced really nice clear bands on the western blot at 110Kda MW. I would highly recommend using this if you are measuring ITGB3.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse blood