Mouse IFN-gamma ELISA Kit

Sensitivity

5.5 pg/mL

Range

31.25-2000 pg/mL

Reactivity

Mouse

Cat no : KE10001

Synonyms

Ifn, IFN g, IFN gamma, Ifng, IFN-gamma, IFN-γ, interferon gamma



Product Information

KE10001 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IFN-gamma ELISA kit is to be used to detect and quantify protein levels of endogenous IFN-gamma. The assay recognizes mouse IFN-gamma. An antibody specific for IFN-gamma has been pre-coated onto the microwells. The IFN-gamma protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for IFN-gamma is added to detect the captured IFN-gamma protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Mouse IFN-gamma ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants
Assay type Sandwich
Sensitivity 5.5 pg/mL
Range 31.25-2000 pg/mL
Reactivity Mouse
Tested applications Sandwich ELISA
Gene ID (NCBI) 15978

Recovery

Sample TypeAverageRange
Mouse serum91%81%-95%
Cell culture supernatants92%79%-104%

IntraAssay

Samplenmean ( pg/mL)SDCV%
120207.110.35.0
220367.416.54.5
3201,101.376.16.9

InterAssay

Samplenmean ( pg/mL)SDCV%
124184.415.08.2
224330.732.49.8
324917.888.99.7

Background Information

The IFNs were originally discovered as agents that interfere with viral replication. Initially, they were classified by the secreting cell type but are now classified into type I and type II according to receptor specificity and sequence homology. Interferon gamma (IFN-γ) is a soluble cytokine that is the only member of the type II class of interferons. It is secreted by Th1 cells, cytotoxic T cells and NK cells. The cytokine is associated with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. It plays crucial roles in pathogen clearance. Aberrant Ifng expression is associated with a number of autoinflammatory and autoimmune diseases. It has been identified in many studies as a biomarker for pleural tuberculosis (TB). Mutations in this gene are associated with aplastic anemia.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms Ifn, IFN g, IFN gamma, Ifng, IFN-gamma, IFN-γ, interferon gamma

Publications

SpeciesSample TypeTitle
mousecell culture supernatants

ACS Nano

Self-Assembly of Podophyllotoxin-Loaded Lipid Bilayer Nanoparticles for Highly Effective Chemotherapy and Immunotherapy via Downregulation of Programmed Cell Death Ligand 1 Production.

Authors - Rui Wang
mousecell culture supernatants

Biomaterials

The programmed site-specific delivery of LY3200882 and PD-L1 siRNA boosts immunotherapy for triple-negative breast cancer by remodeling tumor microenvironment.

Authors - Pan Zhang
mouseserum

Theranostics

Ultrasound (US)-activated redox dyshomeostasis therapy reinforced by immunogenic cell death (ICD) through a mitochondrial targeting liposomal nanosystem

Authors - Junjie Ren
mousecell culture supernatants

Cancer Lett

SIGLEC15 amplifies immunosuppressive properties of tumor-associated macrophages in pancreatic cancer.

Authors - Tian-Jiao Li
mouse

Oxid Med Cell Longev

Simvastatin Reduces NETosis to Attenuate Severe Asthma by Inhibiting PAD4 Expression

Authors - Yun-Rong Chen
mousecorneal allografts

Am J Transplant

The NLRP3 inflammasome regulates corneal allograft rejection through enhanced phosphorylation of STAT3.

Authors - Chao Wei