• Featured Product
  • KD/KO Validated

PDIA6 Polyclonal antibody

PDIA6 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 18233-1-AP

Synonyms

ER protein 5, ERP5, P5, PDIA6, Protein disulfide isomerase A6, Protein disulfide isomerase P5, TXNDC7



Tested Applications

Positive WB detected inrat kidney tissue, mouse heart tissue, mouse liver tissue, mouse kidney tissue, mouse lung tissue, HeLa cells, L02 cells, HEK-293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman stomach tissue, human spleen tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PDIA6 Ready-To-Use IHC Kit. PDIA6 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 18233-1-AP is PDIA6 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PDIA6 fusion protein Ag12940 相同性解析による交差性が予測される生物種
Full Name protein disulfide isomerase family A, member 6
Calculated molecular weight 48 kDa, 54 kDa
Observed molecular weight 48-54 kDa
GenBank accession numberBC001312
Gene symbol PDIA6
Gene ID (NCBI) 10130
RRIDAB_10805765
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PDIA6(Protein disulfide-isomerase A6) is also named as ERP5, P5, TXNDC7 and belongs to the protein disulfide isomerase family.It is one of the endoplasmic reticulum (ER) resident proteins that catalyze formation, reduction, and isomerization of disulfide bonds in proteins and are thought to play a role in folding of disulfide-bonded proteins(PMID:7590364).It has 2 isoforms produced by alternative splicing.This antibody is specific to PDIA6.

Protocols

Product Specific Protocols
WB protocol for PDIA6 antibody 18233-1-APDownload protocol
IHC protocol for PDIA6 antibody 18233-1-APDownload protocol
IF protocol for PDIA6 antibody 18233-1-APDownload protocol
IP protocol for PDIA6 antibody 18233-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Cancer Cell

p53 Is a Master Regulator of Proteostasis in SMARCB1-Deficient Malignant Rhabdoid Tumors.

Authors - Alessandro Carugo
humanWB

Cell Res

Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2.

Authors - Shaojun Zhang
mouseWB

J Exp Med

Essential cell-extrinsic requirement for PDIA6 in lymphoid and myeloid development.

Authors - Jin Huk Choi
humanWB

Nucleic Acids Res

Melanoma RBPome identification reveals PDIA6 as an unconventional RNA-binding protein involved in metastasis.

Authors - Neus Mestre-Farràs
  • KD Validated
humanWB

J Immunother Cancer

MUC1-C is a master regulator of MICA/B NKG2D ligand and exosome secretion in human cancer cells

Authors - Yoshihiro Morimoto
humanWB,IF,IP

Antiviral Res

Venezuelan equine encephalitis virus E1 protein interacts with PDIA6 and PDI inhibition reduces alphavirus production

Authors - Lauren Panny