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P62,SQSTM1 Polyclonal antibody

P62,SQSTM1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (9)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 18420-1-AP

Synonyms

P62, P62/SQSTM1, sequestosome 1, SQSTM1, Ubiquitin binding protein p62



Tested Applications

Positive WB detected inHeLa cells, U2OS cells, HEK-293 cells, HepG2 cells, U-87 MG cells, Jurkat cells
Positive IP detected inHepG2 cells, U2OS cells
Positive IHC detected inhuman liver cancer tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inChloroquine treated HepG2 cells, U2OS cells, Chloroquine treated HeLa cells
Positive FC detected inHEK-293 cells
Planning an IF experiment? We recommend our CoraLite® Plus 647 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:750-1:3000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

18420-1-AP targets P62,SQSTM1 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, goat, chicken, goslings, monkey, rabbit, zebrafish, hamster, pig, duck
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen P62,SQSTM1 fusion protein Ag13131 相同性解析による交差性が予測される生物種
Full Name sequestosome 1
Calculated molecular weight 48 kDa
Observed molecular weight 62 kDa
GenBank accession numberBC017222
Gene symbol SQSTM1
Gene ID (NCBI) 8878
RRIDAB_10694431
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Background

 P62 (ubiquitin-binding protein P62), also known as Sequestosome-1, is a multifunctional adaptor protein most widely known for its role as an autophagosome cargo protein (PMID: 8551575). P62 via specific interactions with polyubiquitylated target proteins induces their selective autophagy (PMID: 17580304). It also plays an important role in the regulation of the NFkB signaling pathway, senescence, cell differentiation, apoptosis, and immune responses (PMID: 26404812).

 What is the molecular weight of P62?

 The observed molecular weight of the protein can vary from as low as 8 kDa (for the smallest isoforms) to 48 kDa.

 What is the subcellular localization of P62?

 P62 is mainly localized in the cytoplasm; however, upon autophagy induction, e.g., via starvation or selective inhibitor treatment, it localizes in vesicular structures - autophagosomes.

 What is the tissue specificity of P62?

 It is ubiquitously expressed in various tissues.

 What is the function of P62 in the regulation of cell death and autophagy?

 It is a selective autophagy receptor that forms a bridge between polyubiquitylated cargo (via its UBA domains) and an autophagy modifier such as LC3 (via LIR domains) (PMIDs: 16286508, 20168092, 24128730, 28404643, 22622177). The process of selective autophagy is tightly regulated at many levels, including the posttranslational modifications (PTMs) of various proteins in the cascade, P62 among others (PMID: 29233872). P62 is involved in the regulation of cell death induction in response to various stimuli, e.g., via activation of caspase-8 at the autophagosome membrane (PMID: 29480462). In addition, P62 is degraded during the autophagic process, which makes its intracellular level a marker for autophagy progression.

 What is P62's involvement in disease?

 Mutations in P62 have been associated with the following diseases: sporadic and familial Paget's disease of bone, neurodegenerative diseases, diabetes, and obesity (PMID: 29480462). A growing number of reports suggest the implication of P62 in the induction of multiple cellular oncogenic transformations. Indeed, increased levels of P62 have been linked to tumor formation, cancer promotion, and resistance to therapy (PMID: 29738493). Moreover, P62 is an unfavorable prognostic marker in liver cancer. 


Protocols

Product Specific Protocols
WB protocol for P62,SQSTM1 antibody 18420-1-APDownload protocol
IHC protocol for P62,SQSTM1 antibody 18420-1-APDownload protocol
IF protocol for P62,SQSTM1 antibody 18420-1-APDownload protocol
IP protocol for P62,SQSTM1 antibody 18420-1-APDownload protocol
FC protocol for P62,SQSTM1 antibody 18420-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

FBXO38 mediates PD-1 ubiquitination and regulates anti-tumour immunity of T cells.

Authors - Xiangbo Meng
humanWB

Signal Transduct Target Ther

Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy

Authors - Yang Meng
humanWB

Cancer Cell

iASPP Is an Antioxidative Factor and Drives Cancer Growth and Drug Resistance by Competing with Nrf2 for Keap1 Binding.

Authors - Wenjie Ge
WB

Cell Metab

Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans.

Authors - Xiao Wang
humanWB

Protein Cell

ORP8 acts as a lipophagy receptor to mediate lipid droplet turnover

Authors - Maomao Pu
WB

Cell Res

NudCL2 is an autophagy receptor that mediates selective autophagic degradation of CP110 at mother centrioles to promote ciliogenesis.

Authors - Min Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

David (Verified Customer) (01-02-2024)

Used for WB in cell lysates. Prominent band at the predicted molecular weight, although some non-specific bands in the control at around 40kDa. However, the correct SQSTM1 band can be clearly visualised.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: SH-SY5Y lysate
FH

Alexandru (Verified Customer) (11-21-2023)

Great at detecting p62 in cell lysates.

  • Applications: Western Blot
  • Cell Tissue Type: HEK-293
FH

Wan (Verified Customer) (02-24-2022)

Perfect antibody, clear bands!

  • Applications: Western Blot
FH

X (Verified Customer) (01-18-2021)

It is a reliable Ab to detect p62 with mouse brain lysate, although there is other band detected.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: mouse brain
P62,SQSTM1 Antibody Western Blot validation (1:2000 dilution) in mouse brain (Cat no:18420-1-AP)
FH

X (Verified Customer) (01-18-2021)

It is a reliable Ab to detect p62 with mouse brain lysate in WB, although there is other bands seen.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: mouse brain
FH

Wei (Verified Customer) (01-30-2020)

Good, Clear band for mouse heart

  • Applications: Western Blot,
  • Cell Tissue Type: Mouse Heart
FH

Joleen (Verified Customer) (06-16-2019)

Recognizes SQSTM1 prominently with some non-specific bands.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1k
  • Cell Tissue Type: MDCK GII
P62,SQSTM1 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:18420-1-AP)