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XBP1S-specific Polyclonal antibody

Name: XBP1S-specific Polyclonal antibody
Brand: Proteintech
SKU: 24868-1-AP
Rating: 5.00 (1 reviews)

XBP1S-specific Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

Reviews (1)

Cat no : 24868-1-AP

Synonyms

TREB5, X box binding protein 1, XBP 1, XBP1, XBP1S, XBP1S-specific, XBP2

Validation Data Gallery

Applications:

AllWBIHCFC

WB analysis using 24868-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 24868-1-AP (XBP1S-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 24868-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 24868-1-AP (XBP1S-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 24868-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 24868-1-AP (XBP1S-specific antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 24868-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 24868-1-AP (XBP1S-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human breast cancer using 24868-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24868-1-AP (XBP1S antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 24868-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24868-1-AP (XBP1S antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 24868-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24868-1-AP (XBP1S-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 24868-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24868-1-AP (XBP1S antibody at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 24868-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24868-1-AP (XBP1S antibody at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 24868-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24868-1-AP (XBP1S antibody at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 24868-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24868-1-AP (XBP1S antibody at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 24868-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 24868-1-AP (XBP1S antibody at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 24868-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 24868-1-AP (XBP1S antibody at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
FC experiment of Ramos using 24868-1-AP
1X10^6 Ramos cells were intracellularly stained with 0.4 ug Anti-Human XBP1S-specific (24868-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Cat No. 24868-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293T cells, MCF-7 cells, mouse liver tissue, rat liver tissue
Positive IHC detected in	human breast cancer tissue, human colon cancer tissue, human liver cancer tissue, mouse liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive FC detected in	Ramos cells

Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Flow Cytometry (FC)	FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Published Applications

KD/KO	See 3 publications below
WB	See 58 publications below
IHC	See 9 publications below
IF	See 7 publications below

Product Information

24868-1-AP targets XBP1S-specific in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, rat, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	XBP1S-specific fusion protein Ag21703 相同性解析による交差性が予測される生物種
Full Name	X-box binding protein 1
Calculated molecular weight	261 aa, 29 kDa
Observed molecular weight	55-65 kDa
GenBank accession number	BC000938
Gene symbol	XBP1
Gene ID (NCBI)	7494
RRID	AB_2879766
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

XBP1, also named as XBP2, belongs to the bZIP family. The X-box-binding protein-1 (XBP1) is a transcriptional regulator of the ER stress response that lies downstream of inositol-requiring enzyme 1 (IRE1α) activation (PMID: 14559994). XBP1 localizes in the nucleus. As a transcription factor, it is essential for hepatocyte growth, the differentiation of plasma cells, the immunoglobulin secretion, and the unfolded protein response. XBP1 has an association with major affective disorder. The unspliced XBP1U isoform is composed of 261 amino acid residues, and the spliced XBP1S isoform is composed of 376 amino acid residues. The calculated molecular weight of protein generated from the unspliced XBP1 isoform XBP1U mRNA is 33 kDa, and protein generated from spliced XBP1 isoform XBP1S mRNA is 54 kDa. This antibody specially recognizes the XBP1S isoform.

Protocols

Product Specific Protocols
WB protocol for XBP1S-specific antibody 24868-1-AP	Download protocol
IHC protocol for XBP1S-specific antibody 24868-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Acta Pharm Sin B The substitution of SERCA2 redox cysteine 674 promotes pulmonary vascular remodeling by activating IRE1α/XBP1s pathway. Authors - Weimin Yu View Article
mouse	WB	Nat Commun Activation of GCN2/ATF4 signals in amygdalar PKC-δ neurons promotes WAT browning under leucine deprivation. Authors - Feixiang Yuan View Article
human	WB	Cancer Lett LIMK1 m6A-RNA methylation recognized by YTHDC2 induces 5-FU chemoresistance in colorectal cancer via endoplasmic reticulum stress and stress granule formation Authors - Lu Chen View Article
	WB	Diabetes Lipid Droplets Protect Human β-Cells From Lipotoxicity-Induced Stress and Cell Identity Changes. Authors - Xin Tong View Article
human	WB	Oxid Med Cell Longev Drp1-Mediated Mitochondrial Metabolic Dysfunction Inhibits the Tumor Growth of Pituitary Adenomas. Authors - Kexia Fan View Article
human	WB,IHC	Oxid Med Cell Longev Deletion of ACLY Disrupts Histone Acetylation and IL-10 Secretion in Trophoblasts, Which Inhibits M2 Polarization of Macrophages: A Possible Role in Recurrent Spontaneous Abortion. Authors - Xin Chen View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

Kevin (Verified Customer) (08-19-2019)

Good antibody. Clear and sharp bands on liver tissue from mouse.

Applications: Western Blot,
Primary Antibody Dilution: 1:1000
Cell Tissue Type: Mouse liver tissue

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

XBP1S-specific Polyclonal antibody

XBP1S-specific Polyclonal Antibody for FC, IHC, WB, ELISA

Validation Data Gallery

WB analysis using 24868-1-AP

WB analysis using 24868-1-AP

WB analysis using 24868-1-AP

WB analysis of mouse liver using 24868-1-AP

IHC staining of human breast cancer using 24868-1-AP

IHC staining of human breast cancer using 24868-1-AP

IHC staining of human liver cancer using 24868-1-AP

IHC staining of human colon cancer using 24868-1-AP

IHC staining of human colon cancer using 24868-1-AP

IHC staining of human liver cancer using 24868-1-AP

IHC staining of human liver cancer using 24868-1-AP

IHC staining of mouse liver using 24868-1-AP

IHC staining of mouse liver using 24868-1-AP

FC experiment of Ramos using 24868-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

The substitution of SERCA2 redox cysteine 674 promotes pulmonary vascular remodeling by activating IRE1α/XBP1s pathway.

Activation of GCN2/ATF4 signals in amygdalar PKC-δ neurons promotes WAT browning under leucine deprivation.

LIMK1 m6A-RNA methylation recognized by YTHDC2 induces 5-FU chemoresistance in colorectal cancer via endoplasmic reticulum stress and stress granule formation

Lipid Droplets Protect Human β-Cells From Lipotoxicity-Induced Stress and Cell Identity Changes.

Drp1-Mediated Mitochondrial Metabolic Dysfunction Inhibits the Tumor Growth of Pituitary Adenomas.

Deletion of ACLY Disrupts Histone Acetylation and IL-10 Secretion in Trophoblasts, Which Inhibits M2 Polarization of Macrophages: A Possible Role in Recurrent Spontaneous Abortion.

Reviews