GFP tag 抗体 57 文献

Mouse Monoclonal| カタログ番号: 66002-1-Ig |クローン番号: 1E10H7

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20,000円

(希望販売価格)

容量: 213 μg/150 μl

Choose quantity:

販売および在庫状況はコスモ・バイオ(株)にお問い合わせください。
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種特異性:
recombinant protein, human

WB ポジティブ:
Recombinant Protein

IP ポジティブ:
Transfected HEK-293 cells

IF ポジティブ:
Transfected HEK-293 cells

推奨希釈倍率:
WB : 1:2000-1:16000
IP : 1:1000-1:4000
IF : 1:20-1:200

製品情報


由来:
Mouse

精製方法:
Protein A purification

アイソタイプ:
IgG2a

バッファー/保存方法:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC.

ターゲット情報


正式名:
GFP tag

計算分子量:
26 kDa

検出分子量:

GenBank accession number:

Gene ID (NCBI):

遺伝子シンボル:

別名:
CFP, eGFP, eYFP, GFP, GFP tag, YFP
背景説明

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Green fluorescence protein(GFP) is a protein composed of 238 amino acid residues(26.9kDa) derived from the Jellyfish Aequorea victoria, which emits green light(emission peak at 509nm) when excited by blue light(excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. EGFP contains the double-amino-acid substitutions Phe-64 to Leu and Ser-65 to Thr(previously published as GFPmut1; PMID: 8707053). In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488nm. GFPmut1 fluoresces 35-fold more intensely than wtGFP when excited at 488nm, due to an increase in its extinction coefficient(Em). The antibody recognizes the GFP-tag, eGFP tag, eYFP tag, CFP tag or YFP tag fused to either the amino- or carboxy-terminus of targeted proteins in transfected mammalian cells.


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