Kallikrein 8 Polyclonal antibody

Kallikrein 8 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14232-1-AP

Synonyms

hK8, HNP, Kallikrein 8, kallikrein related peptidase 8, KLK8, Neuropsin, NP, NRPN, Ovasin, PRSS19, Serine protease 19, Serine protease TADG 14, TADG14



Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

14232-1-AP targets Kallikrein 8 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Kallikrein 8 fusion protein Ag5472
Full Name kallikrein-related peptidase 8
Calculated Molecular Weight 28 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC040887
Gene Symbol KLK8
Gene ID (NCBI) 11202
RRIDAB_2134643
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KLK8(Kallikrein-8) is also named as NRPN, PRSS19, TADG14.It is a new member of the human kallikrein family, which is predicted to be secreted and expected to be present in biological fluids (PMID:12507964).The protein is detectable in ovarian cancer tissue extracts, serum, and ascites fluid, indicating that it may serve as a new ovarian cancer marker (PMID:12782581). KLK8 has one predicted glycosylation site (PMID:20940292) and it has some isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for Kallikrein 8 antibody 14232-1-APDownload protocol
IHC protocol for Kallikrein 8 antibody 14232-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Dis

EZH2-dependent epigenetic modulation of histone H3 lysine-27 contributes to psoriasis by promoting keratinocyte proliferation.

Authors - Tongmei Zhang