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TUBGCP3 Polyclonal antibody

TUBGCP3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 15719-1-AP

Synonyms

GCP 3, GCP3, h104p, hGCP3, hGrip104, hSpc98, SPBC98, Spc98p, TUBGCP3



Tested Applications

Positive WB detected inJurkat cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15719-1-AP targets TUBGCP3 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TUBGCP3 fusion protein Ag8437
Full Name tubulin, gamma complex associated protein 3
Calculated Molecular Weight 103.5 kDa
Observed Molecular Weight 95-101 kDa
GenBank Accession NumberBC007763
Gene Symbol TUBGCP3
Gene ID (NCBI) 10426
RRIDAB_2211272
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TUBGCP3, also named as γ‐tubulin complex protein 3, localized to the centrosome, is a member of γ-tubulin complex proteins which play key roles in microtubule nucleation and organization. It has been reported that TUBGCP3 rotation can bring γ-tubulin molecules into proximity. 15719-1-AP antibody detects the isoform proteins around 95-101 kDa and 50 kDa in SDS-PAGE.

Protocols

Product Specific Protocols
WB protocol for TUBGCP3 antibody 15719-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Cell Biol

The dynamics of microtubule minus ends in the human mitotic spindle.

Authors - Nicolas Lecland
humanWB

Nat Commun

Modular assembly of the principal microtubule nucleator γ-TuRC.

Authors - Martin Würtz
mouseWB

Nat Commun

Non-centrosomal nucleation mediated by augmin organizes microtubules in post-mitotic neurons and controls axonal microtubule polarity.

Authors - Carlos Sánchez-Huertas
humanWB,IF

J Cell Sci

MZT1 regulates microtubule nucleation by linking γTuRC assembly to adapter-mediated targeting and activation.

Authors - Rosa Ramírez Cota
  • KD Validated
humanWB

J Cell Sci

Retromer/WASH dependent sorting of nutrient transporters requires a multivalent interaction network with ANKRD50.

Authors - Arunas Kvainickas
humanWB

J Neuropathol Exp Neurol

Overexpression and Nucleolar Localization of γ-Tubulin Small Complex Proteins GCP2 and GCP3 in Glioblastoma.

Authors - Eduarda Dráberová