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UGCGL1 Monoclonal antibody

UGCGL1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3E8G10

Cat no : 68462-1-Ig

Synonyms

FLJ23671, FLJ23796, GT, HUGT1, UGCGL1, UGGT, UGGT1, UGT1, UGTR

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UGCGL1 Monoclonal antibody (68462-1-Ig)

    WB analysis using 68462-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68462-1-Ig (UGCGL1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, HepG2 cells, Jurkat cells, human placenta tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68462-1-Ig targets UGCGL1 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen UGCGL1 fusion protein Ag5940
Full Name UDP-glucose ceramide glucosyltransferase-like 1
Calculated Molecular Weight 1531 aa, 175 kDa
Observed Molecular Weight170 kDa
GenBank Accession NumberBC041098
Gene Symbol UGCGL1
Gene ID (NCBI) 56886
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UDP-glucose:glycoprotein glucosyltransferase 1 (UGCGL1) is a central quality control gatekeeper in the mammalian endoplasmic reticulum (ER) and serves as a folding sensor in the calnexin/calreticulin glycoprotein quality control cycle (PMID: 28425917). UGCGL1 acts as a checkpoint in the quality control of the MHC class I antigen presentation pathway (PMID: 21383159). Western blot analysis detected UGCGL1at an apparent molecular mass of 170 kDa.

Protocols

Product Specific Protocols
WB protocol for UGCGL1 antibody 68462-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols