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Beta Tubulin Recombinant antibody

Beta Tubulin Recombinant Antibody for WB, IP, IF, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat, zebrafish and More (1)

Applications

WB, IP, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2O13

Cat no : 80713-1-RR

Synonyms

beta 4, beta tubulin, MC1R, TUBB, TUBB4, Tubulin beta 3 chain, Tubulin beta 4 chain, Tubulin beta III, tubulin, beta 3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Beta Tubulin Recombinant antibody (80713-1-RR)

    WB analysis using 80713-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80713-1-RR (Beta Tubulin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of zebrafish tissue using Beta Tubulin Recombinant antibody (80713-1-RR)

    WB analysis of zebrafish using 80713-1-RR

    zebrafish tissue were subjected to SDS PAGE followed by western blot with 80713-1-RR (Beta Tubulin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using Beta Tubulin Recombinant antibody (80713-1-RR)

    IP experiment of HEK-293 using 80713-1-RR

    IP result of anti-Beta Tubulin (IP:80713-1-RR, 4ug; Detection:66240-1-Ig 1:10000) with HEK-293 cells lysate 1360 ug.

  • Immunohistochemistry (IHC) staining of rat brain tissue using Beta Tubulin Recombinant antibody (80713-1-RR)

    IHC staining of rat brain using 80713-1-RR

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80713-1-RR (Beta Tubulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using Beta Tubulin Recombinant antibody (80713-1-RR)

    IHC staining of rat brain using 80713-1-RR

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80713-1-RR (Beta Tubulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of C2C12 cells using Beta Tubulin Recombinant antibody (80713-1-RR)

    IF Staining of C2C12 using 80713-1-RR

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using Beta Tubulin antibody (80713-1-RR, Clone: 2O13 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Beta Tubulin Recombinant antibody (80713-1-RR)

    IF Staining of HeLa using 80713-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Beta Tubulin antibody (80713-1-RR, Clone: 2O13 ) at dilution of 1:550 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HEK-293 cells using Beta Tubulin Recombinant antibody (80713-1-RR)

    FC experiment of HEK-293 using 80713-1-RR

    1X10^6 HEK-293 cells were intracellularly stained with 0.2 ug Anti-Human Beta Tubulin (80713-1-RR, Clone:2O13) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inHeLa cells, zebrafish tissue, HEK-293 cells, Jurkat cells, K-562 cells, Hsc-T6 cells, NIH/3T3 cells, mouse brain, rat brain
Positive IP detected inHEK-293 cells
Positive IF detected inC2C12 cells, HeLa cells
Positive FC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:250-1:1000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

80713-1-RR targets Beta Tubulin in WB, IP, IF, FC, ELISA applications and shows reactivity with Human, mouse, rat, zebrafish samples.

Tested Reactivity Human, mouse, rat, zebrafish
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Beta Tubulin fusion protein Ag0117
Full Name tubulin, beta 3
Calculated Molecular Weight 450 aa, 50 kDa
Observed Molecular Weight50-55 kDa
GenBank Accession NumberBC000748
Gene Symbol TUBB3
Gene ID (NCBI) 10381
RRIDAB_2918906
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha, beta, and gamma tubulins have all been used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs.

Protocols

Product Specific Protocols
WB protocol for Beta Tubulin antibody 80713-1-RRDownload protocol
IHC protocol for Beta Tubulin antibody 80713-1-RRDownload protocol
IF protocol for Beta Tubulin antibody 80713-1-RRDownload protocol
IP protocol for Beta Tubulin antibody 80713-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cells

Fabrication Scaffold with High Dimensional Control for Spheroids with Undifferentiated iPS Cell Properties

Authors - Hidetaka Togo
View Article