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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated ERp72 Polyclonal antibody

ERp72 Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-14712

Synonyms

ER protein 70, ER protein 72, ERp 72, ERP70, ERP72, PDIA4, Protein disulfide isomerase A4

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using CoraLite® Plus 488-conjugated ERp72 Polyclonal ant (CL488-14712)

    IF Staining of PC-3 using CL488-14712

    Immunofluorescent analysis of (-20°C Methanol) fixed PC-3 cells using CoraLite® Plus 488 ERp72 antibody (CL488-14712) at dilution of 1:200.

Tested Applications

Positive IF detected inPC-3 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-14712 targets ERp72 in IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ERp72 fusion protein Ag6431
Full Name protein disulfide isomerase family A, member 4
Calculated Molecular Weight 73 kDa
GenBank Accession NumberBC000425
Gene Symbol PDIA4
Gene ID (NCBI) 9601
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDIA4(Protein disulfide-isomerase A4) is also named as ERP70, ERP72 and belongs to the protein disulfide isomerase family. It catalyzes the rearrangement of -S-S- bonds in proteins. ERp72 is a soluble protein localized in the ER lumen and contains the COOH-terminal retention signal, KEEL. There are 6 cysteine residues in the amino acid sequences of mouse and human ERp72. All of the cysteine residues occur in the internal thioredoxin motif, CGHC(PMID:15475357). The full length protein has a signal peptide with 20 amino acid.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 ERp72 antibody CL488-14712Download protocol
Standard Protocols
Click here to view our Standard Protocols