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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated ACTC1-specific Monoclonal antibody

ACTC1-specific Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1F2B9

Cat no : CL488-66125

Synonyms

ACTC, ACTC1, ACTC1 cardiac muscle 1, ACTC1-specific, Actin, alpha cardiac muscle 1, actin, alpha, cardiac muscle 1, Alpha cardiac actin, CMD1R, CMH11

Validation Data Gallery

Applications:
  • IF Staining of mouse heart using CL488-66125

    IF Staining of mouse heart using CL488-66125

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CL488-66125 (ACTC1-specific antibody) at dilution of 1:100.

  • IF Staining of mouse heart using CL488-66125

    IF Staining of mouse heart using CL488-66125

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CL488-66125 (ACTC1-specific antibody) at dilution of 1:100.

Tested Applications

Positive IF detected inmouse heart tissue
Planning an IHC experiment? We recommend our IHCeasy ACTC1-specific Ready-To-Use IHC Kit. ACTC1-specific primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66125-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-66125 targets ACTC1-specific in IF applications and shows reactivity with human, mouse, pig, rat samples.

Tested Reactivity human, mouse, pig, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name actin, alpha, cardiac muscle 1
Calculated molecular weight 42 kDa
GenBank accession numberNM_005159
Gene symbol ACTC1
Gene ID (NCBI) 70
RRIDAB_2883266
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. The ACTC1 gene encodes cardiac muscle alpha-actin, the predominant actin isoform in adult heart, which interacts with a variety of proteins to produce the force for muscle contraction. This antibody is specific to the ACTC1. It does not cross-react with other actin isoforms. The antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 ACTC1-specific antibody CL488-66125Download protocol
Standard Protocols
Click here to view our Standard Protocols