CoraLite®594-conjugated ACTC1-specific Monoclonal antibody

ACTC1-specific Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1F2B9

Cat no : CL594-66125

Synonyms

ACTC, ACTC1, ACTC1 cardiac muscle 1, ACTC1-specific, Actin, alpha cardiac muscle 1, actin, alpha, cardiac muscle 1, Alpha cardiac actin, CMD1R, CMH11



Tested Applications

Positive IF detected inmouse heart tissue
Positive FC detected inC2C12 cells
Planning an IHC experiment? We recommend our IHCeasy ACTC1-specific Ready-To-Use IHC Kit. ACTC1-specific primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66125-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL594-66125 targets ACTC1-specific in IF, FC (Intra) applications and shows reactivity with human, mouse, pig, rat samples.

Tested Reactivity human, mouse, pig, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name actin, alpha, cardiac muscle 1
Calculated molecular weight 42 kDa
GenBank accession numberNM_005159
Gene symbol ACTC1
Gene ID (NCBI) 70
RRIDAB_2883519
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. The ACTC1 gene encodes cardiac muscle alpha-actin, the predominant actin isoform in adult heart, which interacts with a variety of proteins to produce the force for muscle contraction. This antibody is specific to the ACTC1. It does not cross-react with other actin isoforms. The antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.

Protocols

Product Specific Protocols
IF protocol for CL594 ACTC1-specific antibody CL594-66125Download protocol
FC protocol for CL594 ACTC1-specific antibody CL594-66125Download protocol
Standard Protocols
Click here to view our Standard Protocols