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- KD/KO Validated
HRP-conjugated BID Monoclonal antibody
BID Monoclonal Antibody for WB
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
WB
Conjugate
HRP
CloneNo.
2A2E5
Cat no : HRP-60301
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive WB detected in | Jurkat cells |
Planning an IHC experiment? We recommend our IHCeasy BID Ready-To-Use IHC Kit. BID primary antibody included.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:20000 |
| Sample-dependent, check data in validation data gallery | |
Product Information
HRP-60301 targets BID in WB applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | BID fusion protein Ag21005 相同性解析による交差性が予測される生物種 |
| Full Name | BH3 interacting domain death agonist |
| Calculated molecular weight | 22 kDa |
| Observed molecular weight | 22 kDa |
| GenBank accession number | BC009197 |
| Gene symbol | BID |
| Gene ID (NCBI) | 637 |
| RRID | AB_2883832 |
| Conjugate | HRP |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
BID, also named as p22 BID, can be cleaved into p11 BID, p13 BID and p15 BID. It is pro-apoptotic molecules. The major proteolytic product p15 BID allows the release of cytochrome c. BID-L, BID-EL and BID-ES induce ICE-like proteases and apoptosis. BID-S does not induce apoptosis. BID counters the protective effect of Bcl-2. (PMID:14583606).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HRP BID antibody HRP-60301 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
