CD23 Polyclonal antibody
CD23 Polyclonal Antibody for FC, IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, FC,ELISA
Conjugate
Unconjugated
Cat no : 18642-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Raji cells |
Positive IHC detected in | human tonsillitis tissue, human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC detected in | Raji cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
Sample-dependent, check data in validation data gallery |
Product Information
The immunogen of 18642-1-AP is CD23 Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CD23 fusion protein Ag13348 相同性解析による交差性が予測される生物種 |
Full Name | Fc fragment of IgE, low affinity II, receptor for (CD23) |
Calculated molecular weight | 36 kDa |
Observed molecular weight | 38-42 kDa |
GenBank accession number | BC014108 |
Gene symbol | FCER2 |
Gene ID (NCBI) | 2208 |
RRID | AB_2878555 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CD23, also known as low affinity immunoglobulin epsilon Fc receptor, is a transmembrane glycoprotein present on a subpopulation of B lymphocytes in germinal centres, EBV-transformed B-lymphoblastoid cell lines, follicular dendritic cells, and a subpopulation of peripheral blood cells. CD23 has essential roles in the regulation of IgE production and in the differentiation of B-cells.
Protocols
Product Specific Protocols | |
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WB protocol for CD23 antibody 18642-1-AP | Download protocol |
IHC protocol for CD23 antibody 18642-1-AP | Download protocol |
FC protocol for CD23 antibody 18642-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |