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CD44 Polyclonal antibody
CD44 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
WB, Neutralization, IP, IHC, IF, FC, CoIP, ELISA
Conjugate
Unconjugated
282
Cat no : 15675-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | MDA-MB-231 cells, HeLa cells, HUVEC cells, A549 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human lung cancer tissue, human colon cancer tissue, human oesophagus cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells, human colon cancer tissue, A375 cells, HUVEC cells, UCMSCs cells |
Positive FC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:16000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
Sample-dependent, check data in validation data gallery |
Product Information
15675-1-AP targets CD44 in WB, Neutralization, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human, sika deer, Bovine |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CD44 fusion protein Ag8143 相同性解析による交差性が予測される生物種 |
Full Name | CD44 molecule (Indian blood group) |
Calculated molecular weight | 742 aa, 82 kDa |
Observed molecular weight | 80-95 kDa |
GenBank accession number | BC004372 |
Gene symbol | CD44 |
Gene ID (NCBI) | 960 |
RRID | AB_2076198 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CD44 is a type I transmembrane glycoprotein that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands. Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. This protein exists in multiple forms (with molecular weight ranging from 16 kDa to over 200 kDa) generated by alternative RNA splicing and extensive post-translational modifications.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for CD44 antibody 15675-1-AP | Download protocol |
IHC protocol for CD44 antibody 15675-1-AP | Download protocol |
IF protocol for CD44 antibody 15675-1-AP | Download protocol |
IP protocol for CD44 antibody 15675-1-AP | Download protocol |
FC protocol for CD44 antibody 15675-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Adv Sci (Weinh) The Critical Role of The Piezo1/β-catenin/ATF4 Axis on The Stemness of Gli1+ BMSCs During Simulated Microgravity-Induced Bone Loss | ||
Int J Oral Sci LIMP-2 enhances cancer stem-like cell properties by promoting autophagy-induced GSK3β degradation in head and neck squamous cell carcinoma | ||
Acta Pharm Sin B Engineering cannabidiol synergistic carbon monoxide nanocomplexes to enhance cancer therapy via excessive autophagy | ||
Biomaterials Hyaluronan decorated layer-by-layer assembled lipid nanoparticles for miR-181a delivery in glioblastoma treatment |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Kishor (Verified Customer) (06-23-2019) | This antibody works very good at 1:1000 in WB over night at 4o C and 1:200 for IF in cultured cells.
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