CDC23 Polyclonal antibody
CDC23 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, ELISA
Conjugate
Unconjugated
Cat no : 10683-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | MOLT-4 cells, HEK-293 cells, Jurkat cells, K-562 cells, Caco-2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Sample-dependent, check data in validation data gallery | |
Product Information
10683-1-AP targets CDC23 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CDC23 fusion protein Ag1068 相同性解析による交差性が予測される生物種 |
| Full Name | cell division cycle 23 homolog (S. cerevisiae) |
| Calculated molecular weight | 68 kDa |
| Observed molecular weight | 69 kDa |
| GenBank accession number | BC005258 |
| Gene symbol | CDC23 |
| Gene ID (NCBI) | 8697 |
| RRID | AB_2075008 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CDC23(cell division cycle protein 23 homolog) is also named ANAPC8 and belongs to the APC8/CDC23 family. It is a subunit of the anaphase-promoting complex (APC), which functions at the metaphase-to-anaphase transition of the cell cycle and is regulated by spindle checkpoint proteins. It can be phosphorylated and the phosphorylation of Thr-562 occurs specifically during mitosis(PMID:19690332). It has 3 isoforms produced by alternative splicing with the molecular weight of 69 kDa, 17 kDa, and 56 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CDC23 antibody 10683-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
