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  • KD/KO Validated

CNOT1 Polyclonal antibody

CNOT1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, CoIP, chIP, ELISA

Conjugate

Unconjugated

Cat no : 14276-1-AP

Synonyms

AD 005, CCR4 associated factor 1, CDC39, CNOT1, hNOT1, KIAA1007, NOT1, NOT1H



Tested Applications

Positive WB detected inhuman brain tissue, HEK293 cells, mouse kidney tissue, mouse spleen tissue, mouse thymus tissue, HEK-293 cells, mouse brain tissue, mouse heart tissue, HeLa cells
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman heart tissue, human spleen tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy CNOT1 Ready-To-Use IHC Kit. CNOT1 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14276-1-AP targets CNOT1 in WB, IP, IHC, IF, CoIP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CNOT1 fusion protein Ag5623 相同性解析による交差性が予測される生物種
Full Name CCR4-NOT transcription complex, subunit 1
Calculated molecular weight 267 kDa
Observed molecular weight 240-250 kDa, 174 kDa
GenBank accession numberBC040523
Gene symbol CNOT1
Gene ID (NCBI) 23019
RRIDAB_10888627
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CNOT1 is a component of CCR4-NOT protein complex, which is a regulator of RNA polymerase II transcription, acts as a transcription repressor. CCR4-NOT complex could participate in transcription as well as mRNA degradation. It's highly expressed in brain, heart, thymus, but weak in skeletal muscle and colon. CNOT1 undergoes alternative splicing to produce four isoforms.This is a rabbit polyclonal antibody raised against part chain of C-terminal CNOT1 of human origin. CNOT1 exsits as many isoforms and molecular weight of isoforms are 267, 241 and 173 kDa.

Protocols

Product Specific Protocols
WB protocol for CNOT1 antibody 14276-1-APDownload protocol
IHC protocol for CNOT1 antibody 14276-1-APDownload protocol
IF protocol for CNOT1 antibody 14276-1-APDownload protocol
IP protocol for CNOT1 antibody 14276-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

DND1 maintains germline stem cells via recruitment of the CCR4-NOT complex to target mRNAs.

Authors - Masashi Yamaji
humanWB

Cell

Roquin promotes constitutive mRNA decay via a conserved class of stem-loop recognition motifs.

Authors - Leppek Kathrin K
mouseWB

Cell

The Mammalian Ribo-interactome Reveals Ribosome Functional Diversity and Heterogeneity.

Authors - Deniz Simsek
humanWB

Nat Cancer

SULT1A1-dependent sulfonation of alkylators is a lineage-dependent vulnerability of liver cancers

Authors - Lei Shi
WB

Mol Cell

An Epstein-Barr virus protein interaction map reveals NLRP3 inflammasome evasion via MAVS UFMylation

Authors - Stephanie Pei Tung Yiu
humanWB

Mol Cell

Mechanism of ribosome-associated mRNA degradation during tubulin autoregulation

Authors - Markus Höpfler
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

aditya (Verified Customer) (10-25-2022)

This is brilliant. We use this for cnot1 pull downs and then use a seperate cnot1 antibody to image on the western. Great for identifying interacting partners.

  • Applications: Western Blot, Protein purification
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Hek293
FH

Tobias (Verified Customer) (10-25-2022)

  • Applications: Western Blot, Immunoprecipitation
FH

Parisa (Verified Customer) (10-17-2022)

good quality, high sensitivity

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Hek293
FH

Will (Verified Customer) (01-31-2019)

good antibody, would use again

  • Applications: Western Blot,