HRP-conjugated DYKDDDDK Tag Monoclonal antibody (Binds to FLAG® tag epitope)
DYKDDDDK Tag Monoclonal Antibody for WB
Host / Isotype
Mouse / IgG2b
Reactivity
recombinant protein and More (1)
Applications
WB
Conjugate
HRP
CloneNo.
8H6A10
Cat no : HRP-66008
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Recombinant protein |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:10000-1:100000 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 11 publications below |
Product Information
HRP-66008 targets DYKDDDDK Tag in WB applications and shows reactivity with recombinant protein samples.
| Tested Reactivity | recombinant protein |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | DYKDDDDK Tag |
| Gene symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2883835 |
| Conjugate | HRP |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DYKDDDDK tag, also known as DDK tag, is a hydrophilic tag for recombinant protein technology with the sequence DYKDYKDDDDK. The structure of DYKDDDDK tag has been optimized for compatibility with the proteins it is attached to and unlikely to denature or inactivated them. For its high hydrophilic nature, the DYKDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag more likely to be accessible to antibodies. This product is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DYKDDDDK tags, no matter it is/they are located at N-terminal, C-terminal or at internal regions. The binding of this product with DYKDDDDK tagged fusion protein does not depend on calcium ion. The clone of this monoclonal antibody hybridoma is 1E7B4, which is different from any other vendors. This antibody is HRP conjugated DYKDDDDK Tag antibody. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HRP DYKDDDDK Tag antibody HRP-66008 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun FAM222A encodes a protein which accumulates in plaques in Alzheimer's disease. | ||
Liver Int The DEAD-box helicase DDX3x ameliorates non-alcoholic fatty liver disease via mTORC1 signalling pathway. | ||
Biochem Biophys Res Commun Vaccination with a DNA vaccine encoding CD317-targeting HBs antigen elicits enhanced immunity in mice. | ||
Biochem Biophys Res Commun Harnessing coronavirus spike proteins' binding affinity to ACE2 receptor through a novel baculovirus surface display system. |
