GAPDH 抗体 776 文献

Mouse Monoclonal| カタログ番号: 60004-1-Ig |クローン番号: 1E6D9

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20,000円

(希望販売価格)

容量: 150 μg/150 μl

Choose quantity:

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distributor

種特異性:
human, mouse, rat, zebrafish, yeast, plant, beagle, cow, Cyprinus carpio, Eelworm, fish, hamster, monkey, moth, pig, Porcine Müller, swine, Xenopus laevis

WB ポジティブ:
HeLa cells, arabidopsis whole plant tissue, Raji cells, HEK-293 cells, HepG2 cells, soybean whole plant tissue, whole Nematode tissue, zebrafish tissue, whole yeast cells

IP ポジティブ:
HeLa Cells

IHC ポジティブ:
human kidney tissue, human breast cancer tissue

IF ポジティブ:
Ethacrynic acid treated HeLa cells

FC ポジティブ:
HeLa cells

推奨希釈倍率:
WB : 1:5000-1:50000
IP : 1:2000-1:12000
IHC : 1:50-1:500
IF : 1:20-1:200

製品情報


由来:
Mouse

精製方法:
Protein A purification

アイソタイプ:
IgG2b

バッファー/保存方法:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC.

ターゲット情報


正式名:
glyceraldehyde-3-phosphate dehydrogenase

計算分子量:
36 kDa

検出分子量:
36 kDa

GenBank accession number:

Gene ID (NCBI):

遺伝子シンボル:
GAPDH

別名:
G3PD, GAPD, GAPDH, OK/SW cl.12
背景説明

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus post modification such as S-nitrosylation. This antibody is a mouse monoclonal antibody raised against full length GAPDH of human origin. It can recognize the 36kda GAPDH protein in most cells/tissues. Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.


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