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  • Featured Product
  • KD/KO Validated

HO-1/HMOX1 Polyclonal antibody

HO-1/HMOX1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 10701-1-AP

Synonyms

bK286B10, heme oxygenase (decycling) 1, Heme oxygenase 1, HMOX1, HO, HO 1, HO1, HO-1, HO-1/HMOX1, HSP32

Validation Data Gallery

Applications:
  • WB analysis of HeLa using 10701-1-AP

    WB analysis of HeLa using 10701-1-AP

    WB result of HMOX1 antibody (10701-1-AP, 1:3000) with si-Control and si-HMOX1 transfected HeLa cells.

  • WB analysis using 10701-1-AP

    WB analysis using 10701-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10701-1-AP (HO-1/HMOX1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 10701-1-AP

    WB analysis using 10701-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10701-1-AP (HO-1/HMOX1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 10701-1-AP

    WB analysis using 10701-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10701-1-AP (HMOX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 10701-1-AP

    WB analysis using 10701-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10701-1-AP (HO-1/HMOX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 10701-1-AP

    IP experiment of HeLa using 10701-1-AP

    IP result of anti-HO-1/HMOX1 (IP:10701-1-AP, 4ug; Detection:10701-1-AP 1:20000) with HeLa cells lysate 1360 ug.

  • IHC staining of human liver cancer using 10701-1-AP

    IHC staining of human liver cancer using 10701-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10701-1-AP (HMOX1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HO-1/HMOX1 antibody included
  • IHC staining of human liver cancer using 10701-1-AP

    IHC staining of human liver cancer using 10701-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10701-1-AP (HMOX1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HO-1/HMOX1 antibody included
  • IHC staining of human kidney using 10701-1-AP

    IHC staining of human kidney using 10701-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 10701-1-AP (HMOX1 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with HO-1/HMOX1 antibody included
  • IHC staining of human kidney using 10701-1-AP

    IHC staining of human kidney using 10701-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 10701-1-AP (HMOX1 antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with HO-1/HMOX1 antibody included
  • IF Staining of mouse spleen using 10701-1-AP

    IF Staining of mouse spleen using 10701-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using HO-1/HMOX1 antibody (10701-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse spleen using 10701-1-AP

    IF Staining of mouse spleen using 10701-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using HO-1/HMOX1 antibody (10701-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of HeLa using 10701-1-AP

    FC experiment of HeLa using 10701-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human HO-1/HMOX1 (10701-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inHT-1080 cells, HeLa cells, rat spleen tissue, HepG2 cells, mouse liver tissue, rat liver tissue, mouse kidney tissue, mouse spleen tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse spleen tissue
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy HO-1/HMOX1 Ready-To-Use IHC Kit. HO-1/HMOX1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

10701-1-AP targets HO-1/HMOX1 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, goat, chicken, rat, mouse, monkey, pig, Bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HO-1/HMOX1 fusion protein Ag1190 相同性解析による交差性が予測される生物種
Full Name heme oxygenase (decycling) 1
Calculated molecular weight 33 kDa
Observed molecular weight 28-33 kDa
GenBank accession numberBC001491
Gene symbol HMOX1
Gene ID (NCBI) 3162
RRIDAB_2118685
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Heme oxygenase (HMOX1) catalyzes the first and rate-limiting step in the degradation of heme to yield equimolar quantities of biliverdin Ixa, carbon monoxide (CO), and iron. It has 3 isoforms: HO-1 is highly inducible, whereas HO-2 and HO-3 are constitutively expressed (PMID:10194478). Heme oxygenase-1 (HO-1) is expressed in many tissues and vascular smooth muscle cells, and endothelial cells (PMID:15451051) and has been identified as an important endogenous protective factor induced in many cell types by various stimulants, such as hemolysis, infiammatory cytokines,oxidative stress, heat shock, heavy metals, and endotoxin (PMID: 11522663). And the full-length HO-1 is very unstable and susceptible to truncation that generates an inactive, soluble form (28 kDa) (James R. Reed, Pharmacology, 535-568).

Protocols

Product Specific Protocols
WB protocol for HO-1/HMOX1 antibody 10701-1-APDownload protocol
IHC protocol for HO-1/HMOX1 antibody 10701-1-APDownload protocol
IF protocol for HO-1/HMOX1 antibody 10701-1-APDownload protocol
IP protocol for HO-1/HMOX1 antibody 10701-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

SLC25A39 is necessary for mitochondrial glutathione import in mammalian cells.

Authors - Ying Wang
View Article
WB

Cell Metab

Integrative genetic analysis identifies FLVCR1 as a plasma-membrane choline transporter in mammals

Authors - Timothy C Kenny
View Article
mouseWB

Cell Metab

Functional Genomics In Vivo Reveal Metabolic Dependencies of Pancreatic Cancer Cells.

Authors - Xiphias Ge Zhu
View Article
mouseIHC

ACS Nano

Melatonin-Derived Carbon Dots with Free Radical Scavenging Property for Effective Periodontitis Treatment via the Nrf2/HO-1 Pathway

Authors - Xirui Xin
View Article
humanIHC

Sci Transl Med

PTEN status determines chemosensitivity to proteasome inhibition in cholangiocarcinoma.

Authors - Tian-Yi Jiang
View Article
ratWB

Acta Pharm Sin B

A novel PGAM5 inhibitor LFHP-1c protects blood-brain barrier integrity in ischemic stroke.

Authors - Chenglong Gao
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Brice-Emmanuel (Verified Customer) (12-18-2023)

Works perfectly. Band a 28-33 kDa

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Mouse Heart Tissue
HO-1/HMOX1 Antibody Western Blot validation (1/1000 dilution) in Mouse Heart Tissue (Cat no:10701-1-AP)
FH

James (Verified Customer) (11-18-2022)

Very good antibody! Used it for IF staining of paraffin-embedded spleen tissue. HMOX-1 positive cells are in Green and CD45 in red.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1/200
  • Cell Tissue Type: Spleen
HO-1/HMOX1 Antibody Immunofluorescence validation (1/200 dilution) in Spleen (Cat no:10701-1-AP)
FH

Hala (Verified Customer) (07-28-2021)

incubation 1.5 hours at room temperature dilution 1/3000

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/3000
  • Cell Tissue Type: HepG2
HO-1/HMOX1 Antibody Western Blot validation (1/3000 dilution) in HepG2 (Cat no:10701-1-AP)
FH

JIM (Verified Customer) (03-26-2019)

This antibody offers a good detection of HO-1 expression in LPS stimulated macrophages. The HO-1 expression can be observed in the cells after 24h stimulation of LPS.

  • Applications: Western Blot,