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GRP94 Polyclonal antibody

GRP94 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 14700-1-AP

Synonyms

Cell and organelle markers, ECGP, endoplasmic reticulum marker, Endoplasmin, GP96, gp96 homolog, GRP 94, GRP94, HSP90B1, TRA1, Tumor rejection antigen 1



Tested Applications

Positive WB detected inA431 cells, HEK-293 cells, A549 cells, HeLa cells, HepG2 cells, MCF-7 cells, U2OS cells
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman breast cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, HepG2 cells, A431 cells
Positive FC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite® Plus 647 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

14700-1-AP targets GRP94 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GRP94 fusion protein Ag6344 相同性解析による交差性が予測される生物種
Full Name heat shock protein 90kDa beta (Grp94), member 1
Calculated molecular weight 92 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC066656
Gene symbol HSP90B1
Gene ID (NCBI) 7184
RRIDAB_2233347
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HSP90 proteins are highly conserved molecular chaperones, which normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 (GP96 or GRP94) is an endoplasmic reticulum paralogue of the cytosolic HSP90. As a major ER chaperone to mediate the UPR and a master chaperone for Toll-like receptors (TLRs), HSP90b1 chaperones peptides to MHC class I molecules of dendritic cells and other antigen-presenting cells, as well as facilitating the assembly of immunoglobulin. The protein is also involved in many other bio-processes. This antibody was generated against the C-terminal region of full-length HSP90b1.

Protocols

Product Specific Protocols
WB protocol for GRP94 antibody 14700-1-APDownload protocol
IHC protocol for GRP94 antibody 14700-1-APDownload protocol
IF protocol for GRP94 antibody 14700-1-APDownload protocol
IP protocol for GRP94 antibody 14700-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell

A Translocation Pathway for Vesicle-Mediated Unconventional Protein Secretion.

Authors - Min Zhang
humanWB

Sci Adv

Platelet P-selectin initiates cross-presentation and dendritic cell differentiation in blood monocytes.

Authors - Patrick Han
humanWB,IF

Theranostics

Exosomes from mesenchymal stem cells modulate endoplasmic reticulum stress to protect against nucleus pulposus cell death and ameliorate intervertebral disc degeneration in vivo.

Authors - Zhiwei Liao
ratWB

Redox Biol

Exogenous spermine attenuates rat diabetic cardiomyopathy via suppressing ROS-p53 mediated downregulation of calcium-sensitive receptor.

Authors - Yuehong Wang
humanWB

Haematologica

Comparative analysis of ChAdOx1 nCoV-19 and Ad26.COV2.S SARS-CoV-2 vector vaccines.

Authors - Stephan Michalik
mouseWB

Antioxid Redox Signal

PMID: 27565538

Authors - Tianhe Li