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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LIN28 Monoclonal antibody

LIN28 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

4F5G6

Cat no : 60344-1-Ig

Synonyms

CSDD1, LIN 28, lin 28 homolog (C. elegans), Lin 28A, LIN28, Lin28A, Protein lin 28 homolog A, ZCCHC1

Validation Data Gallery

Applications:
  • WB analysis of NCCIT using 60344-1-Ig

    WB analysis of NCCIT using 60344-1-Ig

    NCCIT cell were subjected to SDS PAGE followed by western blot with 60344-1-Ig (LIN28 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of NCCIT using 60344-1-Ig

    WB analysis of NCCIT using 60344-1-Ig

    NCCIT cell were subjected to SDS PAGE followed by western blot with 60344-1-Ig (LIN28 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 60344-1-Ig

    IHC staining of human prostate cancer using 60344-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60344-1-Ig (LIN28 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 60344-1-Ig

    IHC staining of human prostate cancer using 60344-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60344-1-Ig (LIN28 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human prostate cancer using 60344-1-Ig

    IF Staining of human prostate cancer using 60344-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using LIN28 antibody (60344-1-Ig, Clone: 4F5G6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inNCCIT cell
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman prostate cancer tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

60344-1-Ig targets LIN28 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen LIN28 fusion protein Ag2312 相同性解析による交差性が予測される生物種
Full Name lin-28 homolog (C. elegans)
Calculated molecular weight 209 aa, 23 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC028566
Gene symbol LIN28
Gene ID (NCBI) 79727
RRIDAB_2881453
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LIN28 is one of the four key human factors (OCT4, SOX2, NANOG and LIN28) used to reprogram human fibroblasts to an embryonic stem (ES) cell-like state known as the induced pluripotent stem (Ips) cell. Lin28 is a marker of undifferentiated human embryonic stem cells and a cytoplasmic Mrna-binding protein that binds to and enhances the translation of the IGF2 Mrna. LIN28 has also been shown to bind to the let-7 pre-miRNA and block production of the mature let-7 microRNA in mouse embryonic stem cells. The calculated molecular weight of LIN28 is 23 kDa, but the modified LIN28 is about 28 kDa.

Protocols

Product Specific Protocols
WB protocol for LIN28 antibody 60344-1-IgDownload protocol
IHC protocol for LIN28 antibody 60344-1-IgDownload protocol
IF protocol for LIN28 antibody 60344-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncogene

MSC-regulated lncRNA MACC1-AS1 promotes stemness and chemoresistance through fatty acid oxidation in gastric cancer.

Authors - Wanming He
View Article
mouse

J Histochem Cytochem

KIF11 as a Potential Marker of Spermatogenesis Within Mouse Seminiferous Tubule Cross-sections.

Authors - Miki Hara-Yokoyama
View Article