LC3C-Specific Polyclonal antibody

LC3C-Specific Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 18726-1-AP

Synonyms

LC3C, MAP1A/MAP1B LC3 C, MAP1A/MAP1B light chain 3 C, MAP1LC3C



Tested Applications

Positive WB detected inRecombinant protein
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

18726-1-AP targets LC3C-Specific in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name microtubule-associated protein 1 light chain 3 gamma
Calculated molecular weight 17 kDa
GenBank accession numberNM_001004343
Gene symbol MAP1LC3C
Gene ID (NCBI) 440738
RRIDAB_2878557
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LC3C, also named as MAP1LC3C, belongs to the MAP1 LC3 family. It is the human homolog of yeast Atg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. LC3C is produced by the proteolytic cleavage after the conserved C-terminal Gly residue. The antibody is specific to the prepeptide of LC3C.

Protocols

Product Specific Protocols
IHC protocol for LC3C-Specific antibody 18726-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Autophagy

Mass spectrometry proteomics reveals a function for mammalian CALCOCO1 in MTOR-regulated selective autophagy.

Authors - Jonathan A Stefely
humanWB,IP

Front Pharmacol

SC75741, A Novel c-Abl Inhibitor, Promotes the Clearance of TDP25 Aggregates via ATG5-Dependent Autophagy Pathway

Authors - Dongheng Zhou
humanWB, IF

PLoS One

Autophagosome Proteins LC3A, LC3B and LC3C Have Distinct Subcellular Distribution Kinetics and Expression in Cancer Cell Lines.

Authors - Michael I Koukourakis
humanWB

Adv Exp Med Biol

Microtubule-Associated Protein 1 Light Chain 3 (LC3) Isoforms in RPE and Retina.

Authors - Anuradha Dhingra
humanWB

Dev Cell

V-ATPase recruitment to ER exit sites switches COPII-mediated transport to lysosomal degradation

Authors - Yiwei Sun