Mouse IL-15 ELISA Kit

Sensitivity

50.2 pg/mL

Range

250 -8000 pg/mL

Reactivity

Mouse

Cat no : KE10028

Synonyms

AI503618, Il15, IL-15, interleukin 15



Product Information

KE10028 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL-15 ELISA kit is to be used to detect and quantify protein levels of endogenous IL-15. The assay recognizes mouse IL-15. An antibody specific for IL-15 has been pre-coated onto the microwells. The IL-15 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for IL-15 is added to detect the captured IL-15 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Mouse IL-15 ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants
Assay type Sandwich
Sensitivity 50.2 pg/mL
Range 250 -8000 pg/mL
Reactivity Mouse
Tested applications Sandwich ELISA
Gene ID (NCBI) 16168

Recovery

Sample TypeAverageRange
Mouse serum91%81%-97%
Cell culture supernatants110%99%-124%

IntraAssay

Samplenmean ( pg/mL)SDCV%
120323.323.37.2
220837.152.16.2
3203,300.7215.36.5

InterAssay

Samplenmean ( pg/mL)SDCV%
124242.721.08.6
224868.768.07.8
3243,355.6201.96.0

Background Information

Interleukin 15 (Il15) is a cytokine important for the development, maturation, and function of many cells of the immune system including NK, NKT, gammadeltaT, and CD8(+) T cells. Its expression affects homeostasis and function of B cells through NK cell-derived IFNG. Il-15 binds a trimeric Il-15 receptor (Il-15R) complex formed by a specific receptor chain (Il-15Rα) combined with theβandγchains of the IL-2R, acting as signal transducing components. The transgenic mice overexpressing Il15 exhibit an increase in the number of memory CD8+ T cells in a naive state and enhanced protection against bacterial infections. Mice lacking Il15 exhibit impaired protection against a strain of attenuated Mycobacterium.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms AI503618, Il15, IL-15, interleukin 15

Publications

SpeciesSample TypeTitle
mousecell culture supernatants

J Mammary Gland Biol Neoplasia

TGFß1 Stimulates Lymphatic Endothelial Cells to Produce IL7 and IL15, Which Act as Chemotactic Factors for Breast Cancer Cells with Mesenchymal Properties

Authors - Nikolina Giotopoulou