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iNOS Polyclonal antibody
iNOS Polyclonal Antibody for FC, IF, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (3)
Applications
WB, IP, IF, FC, ELISA
Conjugate
Unconjugated
561
Cat no : 18985-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse liver tissue, rat liver tissue |
| Positive IF detected in | HepG2 cells, HeLa cells |
| Positive FC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:4000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 419 publications below |
| IF | See 158 publications below |
| IP | See 1 publications below |
| ELISA | See 2 publications below |
| FC | See 2 publications below |
Product Information
18985-1-AP targets iNOS in WB, IP, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, chicken, rat, mouse, pig, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | nitric oxide synthase 2, inducible |
| Calculated molecular weight | 131 kDa |
| Observed molecular weight | 110-130 kDa, 65-70 kDa |
| GenBank accession number | NM_000625 |
| Gene symbol | NOS2 |
| Gene ID (NCBI) | 4843 |
| RRID | AB_2782960 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NOS2, also named as iNOS and NOS2A, produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. NO is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission, antimicrobial and antitumoral activities. NOS2 is a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. iNOS has a very short half-life due to rapid degradation by calpain. iNOS monomer is a direct substrate of calpain I and can be cleaved by calpain I at the canonical CaM-binding site(503-532aa) of iNOS, and then a ~70 kDa band can be detected by western (PMID:11786228). This antibody is specific to NOS2.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for iNOS antibody 18985-1-AP | Download protocol |
| IF protocol for iNOS antibody 18985-1-AP | Download protocol |
| FC protocol for iNOS antibody 18985-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Adv Mater Biomimetic Immunosuppressive Exosomes that Inhibit Cytokine Storms Contribute to the Alleviation of Sepsis. | ||
Bioact Mater Reprogramming macrophages via immune cell mobilized hydrogel microspheres for osteoarthritis treatments | ||
J Extracell Vesicles Extracellular vesicle-mediated delivery of circDYM alleviates CUS-induced depressive-like behaviours. | ||
Adv Healthc Mater One-Step Synthesis of Multifunctional Chitosan Hydrogel for Full-Thickness Wound Closure and Healing. | ||
Biomed Opt Express Functional OCT angiography reveals early retinal neurovascular dysfunction in diabetes with capillary resolution | ||
Mater Today Bio Robust drug bioavailability and safety for rheumatoid arthritis therapy using D-amino acids-based supramolecular hydrogels. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Yasuyo (Verified Customer) (03-01-2022) | This is one of the best IHC signaling I ever got for iNOS. Amazing image. I recommend this for iNOS detection by IHC.
 |
FH Xie (Verified Customer) (10-06-2021) | Works nice for WB assay.
 |
FH Prasanth (Verified Customer) (08-19-2019) | This is one of the cleanest and clarity blot I ever got for iNOS. Amazing clarity and no non specific bands. I recommend this for iNOS detection by western blot.
|
FH Elena (Verified Customer) (08-14-2019) | We tried this antibody with 2 cell lines and it worked just fine. We will use this in the future for other cell lines as well. The antibody produced strong bands at the suggested concentration and we diluted it further.
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