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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

P2RX5 Polyclonal antibody

P2RX5 Polyclonal Antibody for FC, IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 19012-1-AP

Synonyms

ATP receptor, P2RX5, P2X purinoceptor 5, P2X5, P2X5R, Purinergic receptor

Validation Data Gallery

Applications:
  • WB analysis of BxPC-3 using 19012-1-AP

    WB analysis of BxPC-3 using 19012-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 19012-1-AP (P2RX5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of HL-60 using 19012-1-AP

    WB analysis of HL-60 using 19012-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 19012-1-AP (P2RX5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of MDA-MB-453s using 19012-1-AP

    WB analysis of MDA-MB-453s using 19012-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 19012-1-AP (P2RX5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of BxPC-3 using 19012-1-AP

    IF Staining of BxPC-3 using 19012-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed BxPC-3 cells using 19012-1-AP (P2RX5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of K-562 using 19012-1-AP

    FC experiment of K-562 using 19012-1-AP

    1X10^6 K-562 cells were stained with 0.2ug P2RX5 antibody (19012-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Tested Applications

Positive WB detected inBxPC-3 cells, HL-60 cells, MDA-MB-453s cells
Positive IF detected inBxPC-3 cells
Positive FC detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Published Applications

FCSee 1 publications below

Product Information

19012-1-AP targets P2RX5 in WB, IF, FC,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen P2RX5 fusion protein Ag5297 相同性解析による交差性が予測される生物種
Full Name purinergic receptor P2X, ligand-gated ion channel, 5
Calculated molecular weight 422 aa, 47 kDa
Observed molecular weight 44 kDa, 49 kDa, 116 kDa
GenBank accession numberBC039015
Gene symbol P2RX5
Gene ID (NCBI) 5026
RRIDAB_2299079
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for P2RX5 antibody 19012-1-APDownload protocol
IF protocol for P2RX5 antibody 19012-1-APDownload protocol
FC protocol for P2RX5 antibody 19012-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanFC

PLoS One

A Truncation Variant of the Cation Channel P2RX5 Is Upregulated during T Cell Activation.

Authors - Pierre Abramowski
View Article