Nectin 2 Polyclonal antibody

Nectin 2 Polyclonal Antibody for FC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 10100-2-AP

Synonyms



Tested Applications

Positive WB detected inmouse kidney tissue, 3T3-L1 cells, mouse heart tissue
Positive FC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy Nectin-2/PVRL2 Ready-To-Use IHC Kit. Nectin-2/PVRL2 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Flow Cytometry (FC)FC : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10100-2-AP targets Nectin 2 in WB, IF, FC,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Nectin 2 fusion protein Ag0149 相同性解析による交差性が予測される生物種
Full Name poliovirus receptor-related 2 (herpesvirus entry mediator B)
Calculated molecular weight 58 kDa
GenBank accession numberBC003091
Gene symbol PVRL2
Gene ID (NCBI) 5819
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Poliovirus receptor-related 2 (herpesvirus entry mediator B) (PVRL2, synonyms: HVEB, PRR2, CD112, PVRR2) is an adhesion molecule widely expressed in cell lines of different lineages, including hematopoietic, neuronal, endothelial and epithelial cells. CD112 belongs to a new family of immunoglobulin-like molecules that includes four members (CD111 , CD112, PRR3 and CD155) sharing an ectodomain made of three Ig domains, of V, C, C types. CD112 is expressed in the myelo-monocytic and megakaryocytic hematopoietic lineages and the function in hematopoiesis is currently unknown. CD112 is an intercellular homophilic adhesion. CD112 localizes specifically at adherents junctions via its cytoplasmic interaction with the scaffold F-actin binding protein afadin. This interaction is mediated by a sequence located to the C terminal ends of both isoforms of CD112 (A/ExYV) and the PDZ domain of afadin. This sequence is also found in CD112 and PRR3 and represents a specific consensus in the family, which is also named nectin family. Disruption of the murine CD112 gene leads to infertility of male mice with morphologically aberrant spermatozoa. CD112 mediates entry of some alphaherpesvirus mutants (also named HveB) via its V domain. CD112 is involved in cell to cell spreading of viruses

Publications

SpeciesApplicationTitle
monkeyWB,IF

Eur J Cell Biol

Membrane palmitoylated proteins regulate trafficking and processing of nectins.

Authors - Dudak Amanda A