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RUVBL2 Polyclonal antibody
RUVBL2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 10195-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | C2C12 cells, Daudi cells, HeLa cells, HepG2 cells |
Positive IP detected in | HeLa cells, mouse brain tissue |
Positive IHC detected in | human testis tissue, human kidney tissue, human placenta tissue, human skin tissue, human brain tissue, human heart tissue, human lymphoma tissue, human gliomas tissue, human spleen tissue, human lung tissue, human ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 3 publications below |
WB | See 16 publications below |
IHC | See 2 publications below |
IF | See 2 publications below |
IP | See 2 publications below |
Product Information
10195-1-AP targets RUVBL2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | RUVBL2 fusion protein Ag0253 相同性解析による交差性が予測される生物種 |
Full Name | RuvB-like 2 (E. coli) |
Calculated molecular weight | 51 kDa |
Observed molecular weight | 51 kDa |
GenBank accession number | BC000428 |
Gene symbol | RUVBL2 |
Gene ID (NCBI) | 10856 |
RRID | AB_2184679 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RuvB-like 2 (E. coli) (RUVBL2, synonyms: ECP51, TIP48, CGI-46, TIP49B) is the second human homologue of the bacterial RuvB gene. Bacterial RuvB protein is a DNA helicase essential for homologous recombination and DNA double-strand break repair. Functional analysis showed that RUVBL2 has both ATPase and DNA helicase activities.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for RUVBL2 antibody 10195-1-AP | Download protocol |
IHC protocol for RUVBL2 antibody 10195-1-AP | Download protocol |
IF protocol for RUVBL2 antibody 10195-1-AP | Download protocol |
IP protocol for RUVBL2 antibody 10195-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Mol Cell Metabolic stress controls mTORC1 lysosomal localization and dimerization by regulating the TTT-RUVBL1/2 complex. | ||
Sci Adv Synthetic lethality by targeting the RUVBL1/2-TTT complex in mTORC1-hyperactive cancer cells.
| ||
Nat Commun Inhibition of the CDK2 and Cyclin A complex leads to autophagic degradation of CDK2 in cancer cells. | ||
J Clin Invest circular RNA cia-MAF drives self-renewal and metastasis of liver tumor-initiating cells via transcription factor MAFF. | ||
Sci Adv ETV7 is an essential component of a rapamycin-insensitive mTOR complex in cancer. | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Deyong (Verified Customer) (03-11-2022) | It worked well in H1 human embryonic stem cells.
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