SAE1 Polyclonal antibody

SAE1 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10229-1-AP

Synonyms

AOS1, FLJ3091, HSPC140, SAE1, SUA1, UBLE1A



Tested Applications

Positive WB detected inA431 cells, A549 cells, COLO 320 cells, HEK-293 cells, HeLa cells
Positive IHC detected inhuman colon cancer tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10229-1-AP targets SAE1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SAE1 fusion protein Ag0275 相同性解析による交差性が予測される生物種
Full Name SUMO1 activating enzyme subunit 1
Calculated molecular weight 38 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC000344
Gene symbol SAE1
Gene ID (NCBI) 10055
RRIDAB_2182917
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SAE1(SUMO-activating enzyme subunit 1) is also named as AOS1, SUA1, UBLE1A and belongs to the ubiquitin-activating E1 family. SAE1 has a calculated molecular mass of 38 kD. It had an apparent molecular mass of 40 kD by SDS-PAGE. SAE1 and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins(PMID:9920803). Western blot analysis of synchronized HeLa cells detectes increased AOS1 expression as cells progressed through S phase, followed by a substantial decrease in G2 phase. Immunofluorescence analysis shows AOS1 and UBA2 distributed throughout nuclei, but they are excluded from nucleoli.(PMID:11481243).

Protocols

Product Specific Protocols
WB protocol for SAE1 antibody 10229-1-APDownload protocol
IHC protocol for SAE1 antibody 10229-1-APDownload protocol
IF protocol for SAE1 antibody 10229-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Curr Biol

Pumilio 1 suppresses multiple activators of p53 to safeguard spermatogenesis.

Authors - Dong Chen
humanIHC

Open Med (Wars)

Bioinformatics-based analysis of SUMOylation-related genes in hepatocellular carcinoma reveals a role of upregulated SAE1 in promoting cell proliferation.

Authors - Yang Liu
humanWB

Nat Commun

SENP1 prevents steatohepatitis by suppressing RIPK1-driven apoptosis and inflammation

Authors - Lingjie Yan
humanIHC,WB

Curr Issues Mol Biol

SUMO-Activating Enzyme Subunit 1 Is Associated with Poor Prognosis, Tumor Progression, and Radio-Resistance in Colorectal Cancer

Authors - Yueh-Jung Wu
humanWB

Elife

TRIM28 promotes HIV-1 latency by SUMOylating CDK9 and inhibiting P-TEFb.

Authors - Xiancai Ma