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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SF3A1 Polyclonal antibody

SF3A1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15858-1-AP

Synonyms

PRP21, PRPF21, SAP 114, SAP114, SF3A1, SF3A120, Splicing factor 3A subunit 1

Validation Data Gallery

Applications:
  • WB analysis using 15858-1-AP

    WB analysis using 15858-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15858-1-AP (SF3A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 15858-1-AP

    WB analysis of mouse brain using 15858-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 15858-1-AP (SF3A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of HL-60 using 15858-1-AP

    WB analysis of HL-60 using 15858-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 15858-1-AP (SF3A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 15858-1-AP

    WB analysis of HEK-293 using 15858-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 15858-1-AP (SF3A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 15858-1-AP

    WB analysis of HeLa using 15858-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15858-1-AP (SF3A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse lung using 15858-1-AP

    WB analysis of mouse lung using 15858-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 15858-1-AP (SF3A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 15858-1-AP

    IP experiment of mouse brain using 15858-1-AP

    IP result of anti-SF3A1 (IP:15858-1-AP, 4ug; Detection:15858-1-AP 1:500) with mouse brain tissue lysate 4000ug.

  • IHC staining of human breast cancer using 15858-1-AP

    IHC staining of human breast cancer using 15858-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 15858-1-AP (SF3A1 antibody) at dilution of 1:400 (under 10x lens).

    Ready-to-use IHC kits available with SF3A1 antibody included
  • IHC staining of human breast cancer using 15858-1-AP

    IHC staining of human breast cancer using 15858-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 15858-1-AP (SF3A1 antibody) at dilution of 1:400 (under 40x lens).

    Ready-to-use IHC kits available with SF3A1 antibody included
  • IHC staining of human breast cancer using 15858-1-AP

    IHC staining of human breast cancer using 15858-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 15858-1-AP (SF3A1 antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with SF3A1 antibody included
  • IHC staining of human breast cancer using 15858-1-AP

    IHC staining of human breast cancer using 15858-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 15858-1-AP (SF3A1 antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with SF3A1 antibody included
  • IF Staining of HepG2 using 15858-1-AP

    IF Staining of HepG2 using 15858-1-AP

    Immunofluorescent analysis of HepG2 cells, using SF3A1 antibody 15858-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inHL-60 cells, mouse brain tissue, HEK-293 cells, HeLa cells, mouse lung tissue, K-562 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy SF3A1 Ready-To-Use IHC Kit. SF3A1 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

15858-1-AP targets SF3A1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SF3A1 fusion protein Ag8196 相同性解析による交差性が予測される生物種
Full Name splicing factor 3a, subunit 1, 120kDa
Calculated molecular weight 793 aa, 89 kDa
Observed molecular weight 120-130 kDa
GenBank accession numberBC007684
Gene symbol SF3A1
Gene ID (NCBI) 10291
RRIDAB_2186354
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SF3A1, also known as SF3a120, is a 793 amino acid protein, which is a Subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. SF3A1 also be involved in the assembly of the 'E' complex. The calculated molecular weight of SF3A1 is 88 kDa, but the modified SF3A1 is about 115-120 kDa.(PMID: 7489498 )

Protocols

Product Specific Protocols
WB protocol for SF3A1 antibody 15858-1-APDownload protocol
IHC protocol for SF3A1 antibody 15858-1-APDownload protocol
IF protocol for SF3A1 antibody 15858-1-APDownload protocol
IP protocol for SF3A1 antibody 15858-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Rep

SF3B1 Association with Chromatin Determines Splicing Outcomes.

Authors - Nir Kfir
View Article
mouseWB

Front Pharmacol

LXR agonist inhibits inflammation through regulating MyD88 mRNA alternative splicing

Authors - Ni Li
View Article
humanIP

bioRxiv

Integrative identification of non-coding regulatory regions driving metastatic prostate cancer

Authors - Brian J Woo
View Article