Overview, pathway and related antibodies
Nuclear factor kappa B (NF-κB) is a protein complex that controls transcription of DNA, cytokine production and cell survival. NF-κB is composed of multiple subunits: RelA (p65), RelB, c-Rel, NF-κB1 (p50) and NF-κB2 (p52) (1).
These proteins function as dimeric transcription factors that regulate the expression of genes influencing a broad range of biological processes including innate and adaptive immunity, inflammation, stress responses, B-cell development, and lymphoid organogenesis (2). NF-κB exists freely in the cytoplasm, where it is inhibited by IκB proteins. In the classical (canonical) pathway, NF-κB activation occurs through the phosphorylation, ubiquitination and subsequent proteosomal degradation of IκB. An alternative (non-cononical) activation involves phosphorylation and processing of the p52 precursor, p100 into the mature protein and subsequent nuclear translocation of the RelB/p52, heterodimer that translocate to the nucleus and induce target gene expression (3).
Abnormalities in NF-κB pathway has been linked to cancer, inflammatory and autoimmune diseases, septic shock, viral infection, and improper immune development. NF-κB has also been implicated in processes of synaptic plasticity and memory (4,5).
Download the NF-KB Pathway as a PDF here |
|
| Antibody | Catalog number | Type | Applications | |
| NF-ΚB | 14220-1-AP | Rabbit Poly | ELISA, IF, IHC, IP, WB | 24 Publications |
| P65; RELA - KD/KO Validated | 10745-1-AP | Rabbit Poly | ELISA, FC, IF, IHC, IP, WB | 128 Publications |
| NFKB2 | 10409-2-AP | Rabbit Poly | ELISA, IF, IHC, IP, WB | 3 Publications |
| NFKB1 | 23576-1-AP | Rabbit Poly | ELISA, WB, IHC, IF |
 |
Immunofluorescent analysis of HeLa cells using 14220-1-AP(NF-κB antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG |
References:
