• HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human M-CSF protein

Activity

0.7-4.0 ng/mL EC50

Specific Activity

>4.00 x 107 IU/mg

Species Reactivity

human,mouse,rat

Purity

>95%

Cat no : HZ-1192

Synonyms

CSF 1, CSF1, Lanimostim, M CSF, MCSF, M-CSF



Technical Specifications

GeneID 1435
Species Human
Expression HEK293
Activity 0.7-4.0 ng/mL EC50
Specific Activity>4.00 x 107 IU/mg
Purity >95%
Endotoxin<1 EU/µg
Accession NumberP09603
Molecular Mass 19 and 23 kDa reduced, 35 and 40 kDa non-reduced, homodimer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,mouse,rat

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

M-CSF plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes. M-CSF promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. It also plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. On the cellular level, M-CSF promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration and plays a role in lipoprotein clearance (PMID: 29734839; 29679908; 28202039)

Synonyms

CSF 1, CSF1, Lanimostim, M CSF, MCSF, M-CSF

Publications

SpeciesTitle
mouse

Cell Metab

Senescent immune cells release grancalcin to promote skeletal aging.

Authors - Chang-Jun Li
mouse

Cell Metab

A mechanosensitive lipolytic factor in the bone marrow promotes osteogenesis and lymphopoiesis.

Authors - Hui Peng
mouse

ACS Nano

Bioinspired Nanovesicles Convert the Skeletal Endothelium-Associated Secretory Phenotype to Treat Osteoporosis.

Authors - Yongzhi Cui
mouse

Adv Sci (Weinh)

Exosomal miR-181d-5p Derived from Rapamycin-Conditioned MDSC Alleviated Allograft Rejection by Targeting KLF6

Authors - Chao Wei
human

Autophagy

Induction of a Na+/K+-ATPase-dependent form of autophagy triggers preferential cell death of human immunodeficiency virus type-1-infected macrophages.

Authors - Gang Zhang
mouse

Acta Biomater

Exposure to hydroxyapatite nanoparticles enhances Toll-like receptor 4 signal transduction and overcomes endotoxin tolerance in vitro and in vivo.

Authors - Yuchen Hua