Various lysates were subjected to SDS PAGE followed by western blot with 67808-1-Ig (SMCR7L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67808-1-Ig (SMCR7L antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using SMCR7L antibody (67808-1-Ig, Clone: 3B3G3 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). DAPI (blue).