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  • KD/KO Validated

APPL1 Polyclonal antibody

APPL1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 12639-1-AP

Synonyms

APPL, APPL1, Dip13 alpha, DIP13A, DIP13alpha, KIAA1428



Tested Applications

Positive WB detected inC2C12 cells, HEK-293 cells, mouse ovary tissue, HeLa cells, mouse liver tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

12639-1-AP targets APPL1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen APPL1 fusion protein Ag3334 相同性解析による交差性が予測される生物種
Full Name adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1
Calculated molecular weight 709 aa, 80 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC028599
Gene symbol APPL1
Gene ID (NCBI) 26060
RRIDAB_2289669
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (APPL1), a binding partner of Akt2 and an important regulator of INS signaling, plays a key role in the regulation of INS secretion [PMID:22615370]. APPL1 interacts with adiponectin receptors and mediates the INS-sensitizing effects of adiponectin in muscle and endothelial cells. It also participates in nuclear signaling and transcriptional regulation, mostly by modulating the activity of various nuclear factors [PMID:22685329]. Apart from its role in endocytosis and endosomal transport, APPL1 was reported to undergo nucleocytoplasmic shuttling and participate in transcriptional regulation, e.g. by interactions with histone deacetylases (HDACs) [PMID:19686092].

Protocols

Product Specific Protocols
WB protocol for APPL1 antibody 12639-1-APDownload protocol
IHC protocol for APPL1 antibody 12639-1-APDownload protocol
IF protocol for APPL1 antibody 12639-1-APDownload protocol
IP protocol for APPL1 antibody 12639-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Oxid Med Cell Longev

DDAH1/ADMA Regulates Adiponectin Resistance in Cerebral Ischemia via the ROS/FOXO1/APR1 Pathway.

Authors - Yichen Zhao
humanIF

J Cell Sci

ESCRT-0 marks an APPL1-independent transit route for EGFR between the cell surface and the EEA1-positive early endosome.

Authors - Neftali Flores-Rodriguez
humanWB

J Biol Chem

Endocytosis is required for C-X-C chemokine receptor type 4 (CXCR4)-mediated Akt activation and anti-apoptotic signaling.

Authors - Elizabeth J English
  • KD Validated
mouseWB

J Agric Food Chem

Naringin Promotes Skeletal Muscle Fiber Remodeling by the AdipoR1-APPL1-AMPK Signaling Pathway.

Authors - Peiyuan Li
mouseWB,IF

Biol Reprod

Myosin VI maintains the actin-dependent organization of the tubulobulbar complexes required for endocytosis during mouse spermiogenesis.

Authors - Przemysław Zakrzewski
humanWB,IHC

Brain Res

Characterization and distribution of adaptor protein containing a PH domain, PTB domain and leucine zipper motif (APPL1) in Alzheimer's disease hippocampus: an immunohistochemical study.

Authors - Akari Ogawa

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Ying (Verified Customer) (09-11-2023)

used for WB and IFC of brain section of wild type and APPL1 transgenic mice

  • Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
  • Primary Antibody Dilution: 1:500 for IFC, 1:1000 for WB
  • Cell Tissue Type: mouse brain/human fibroblast
APPL1 Antibody Western Blot,Immunohistochemistry,Immunofluorescence,Immunoprecipitation validation (1:500 for IFC, 1:1000 for WB dilution) in mouse brain/human fibroblast (Cat no:12639-1-AP)
FH

Sophie (Verified Customer) (11-11-2022)

I used the antibody in a western blot, my samples were primary B-cell lysates (10ug/lane). The antibody did label APPL1 around the 80kDa mark, but I did see another band lower down, around 37kDa - maybe this is a cleavage product?

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2,000
  • Cell Tissue Type: Primary B-cells (human, from blood)
APPL1 Antibody Western Blot validation (1:2,000 dilution) in Primary B-cells (human, from blood) (Cat no:12639-1-AP)
FH

X (Verified Customer) (01-18-2021)

Very strong and specific signal in WB with mouse brain lysate

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: mouse brain
APPL1 Antibody Western Blot validation (1:5000 dilution) in mouse brain (Cat no:12639-1-AP)