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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ATP5J2 Monoclonal antibody

ATP5J2 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Rabbit and More (1)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2B3C3

Cat no : 68128-1-Ig

Synonyms

ATP5J2, ATP5JL

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using ATP5J2 Monoclonal antibody (68128-1-Ig)

    WB analysis using 68128-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68128-1-Ig (ATP5J2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ATP5J2 Monoclonal antibody (68128-1-Ig)

    WB analysis using 68128-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68128-1-Ig (ATP5J2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rabbit heart tissue using ATP5J2 Monoclonal antibody (68128-1-Ig)

    WB analysis of rabbit heart using 68128-1-Ig

    rabbit heart tissue were subjected to SDS PAGE followed by western blot with 68128-1-Ig (ATP5J2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ATP5J2 Monoclonal antibody (68128-1-Ig)

    WB analysis using 68128-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68128-1-Ig (ATP5J2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using ATP5J2 Monoclonal antibody (68128-1-Ig)

    IF Staining of MCF-7 using 68128-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using ATP5J2 antibody (68128-1-Ig, Clone: 2B3C3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inrat heart tissue, rabbit heart tissue, LNCaP cells, mouse heart tissue, mouse liver tissue, rat liver tissue, HeLa cells, HepG2 cells
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

68128-1-Ig targets ATP5J2 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit samples.

Tested Reactivity Human, Mouse, Rat, Rabbit
Cited Reactivity mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ATP5J2 fusion protein Ag8811 相同性解析による交差性が予測される生物種
Full Name ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F2
Calculated molecular weight 5-11 kDa
Observed molecular weight12 kDa
GenBank accession numberBC003678
Gene symbol ATP5J2
Gene ID (NCBI) 9551
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ATP5J2 antibody 68128-1-IgDownload protocol
IF protocol for ATP5J2 antibody 68128-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Commun

N-Acetyltransferase 10 represses Uqcr11 and Uqcrb independently of ac4C modification to promote heart regeneration

Authors - Wenya Ma
View Article