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KD/KO Validated

CA12 Polyclonal antibody

Name: CA12 Polyclonal antibody
Brand: Proteintech
SKU: 15180-1-AP

CA12 Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 15180-1-AP

Synonyms

CA XII, CA12, Carbonate dehydratase XII, Carbonic anhydrase 12, carbonic anhydrase XII, CAXII, HsT18816, Tumor antigen HOM RCC 3.1.3

Validation Data Gallery

Applications:

AllWBIHCFC

WB analysis using 15180-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 15180-1-AP (CA12 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 15180-1-AP
WB result of CA12 antibody (15180-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CA12 transfected MCF-7 cells.
WB analysis of A549 using 15180-1-AP
WB result of CA12 antibody (15180-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CA12 transfected A549 cells.
WB analysis of BxPC-3 using 15180-1-AP
BxPC-3 cells were subjected to SDS PAGE followed by western blot with 15180-1-AP (CA12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 15180-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 15180-1-AP (CA12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human pancreas using 15180-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 15180-1-AP (CA12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas using 15180-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 15180-1-AP (CA12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 15180-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 15180-1-AP (CA12 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 15180-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 15180-1-AP (CA12 antibody) at dilution of 1:50 (under 40x lens).
FC experiment of HEK-293 using 15180-1-AP
1X10^6 HEK-293 cells were stained with 0.2ug CA12 antibody (15180-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Cat No. 15180-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A549 cells, BxPC-3 cells, MCF-7 cells
Positive IHC detected in	human pancreas tissue, human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive FC detected in	HEK-293 cells

Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Flow Cytometry (FC)	FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Published Applications

KD/KO	See 1 publications below
WB	See 16 publications below
IHC	See 2 publications below
IF	See 5 publications below
IP	See 1 publications below

Product Information

15180-1-AP targets CA12 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, rat, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CA12 fusion protein Ag7107 相同性解析による交差性が予測される生物種
Full Name	carbonic anhydrase XII
Calculated molecular weight	39 kDa
Observed molecular weight	44 kDa, 75-80 kDa
GenBank accession number	BC000278
Gene symbol	CA12
Gene ID (NCBI)	771
RRID	AB_2065826
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CA12 belongs to the alpha-carbonic anhydrase family. It reversible hydration of carbon dioxide. Overexpression of the zinc enzyme CA12 is observed in certain human cancers. The structure reveals a prototypical CA fold; however, two CA12 domains associate to form an isologous dimer, an observation that is confirmed by studies of the enzyme in solution.

Protocols

Product Specific Protocols
WB protocol for CA12 antibody 15180-1-AP	Download protocol
IHC protocol for CA12 antibody 15180-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB, IF	J Exp Med Large pH oscillations promote host defense against human airways infection. Authors - Dusik Kim View Article
human	WB	Mol Oncol Mechanism of irradiation-induced mammary cancer metastasis: A role for SAP-dependent Mkl1 signaling. Authors - Maria B Asparuhova View Article
human	WB,IF	Hum Mol Genet Loss of carbonic anhydrase XII function in individuals with elevated sweat chloride concentration and pulmonary airway disease. Authors - Melissa Lee View Article
human	IHC	Int J Mol Sci Perfusion-Based Bioreactor Culture and Isothermal Microcalorimetry for Preclinical Drug Testing with the Carbonic Anhydrase Inhibitor SLC-0111 in Patient-Derived Neuroblastoma. Authors - Zihe Huo View Article
human	WB,IF	J Enzyme Inhib Med Chem Carbonic anhydrase 12 mutation modulates membrane stability and volume regulation of aquaporin 5. Authors - Soyoung Hwang View Article
human	WB	Front Oncol Integrative Proteo-Genomic Analysis for Recurrent Survival Prognosis in Colon Adenocarcinoma. Authors - FeiYan Ai View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CA12 Polyclonal antibody

CA12 Polyclonal Antibody for FC, IHC, WB, ELISA

Validation Data Gallery

WB analysis using 15180-1-AP

WB analysis of MCF-7 using 15180-1-AP

WB analysis of A549 using 15180-1-AP

WB analysis of BxPC-3 using 15180-1-AP

WB analysis of PC-3 using 15180-1-AP

IHC staining of human pancreas using 15180-1-AP

IHC staining of human pancreas using 15180-1-AP

IHC staining of human kidney using 15180-1-AP

IHC staining of human kidney using 15180-1-AP

FC experiment of HEK-293 using 15180-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Large pH oscillations promote host defense against human airways infection.

Mechanism of irradiation-induced mammary cancer metastasis: A role for SAP-dependent Mkl1 signaling.

Loss of carbonic anhydrase XII function in individuals with elevated sweat chloride concentration and pulmonary airway disease.

Perfusion-Based Bioreactor Culture and Isothermal Microcalorimetry for Preclinical Drug Testing with the Carbonic Anhydrase Inhibitor SLC-0111 in Patient-Derived Neuroblastoma.

Carbonic anhydrase 12 mutation modulates membrane stability and volume regulation of aquaporin 5.

Integrative Proteo-Genomic Analysis for Recurrent Survival Prognosis in Colon Adenocarcinoma.