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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

EIF3D Polyclonal antibody

EIF3D Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, yeast and More (2)

Applications

WB, PLA, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 10219-1-AP

Synonyms

eIF 3 zeta, eIF3 p66, eIF3 zeta, EIF3D, EIF3S7

Validation Data Gallery

Applications:
  • WB analysis of mouse brain using 10219-1-AP

    WB analysis of mouse brain using 10219-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10219-1-AP (EIF3D Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis using 10219-1-AP

    WB analysis using 10219-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10219-1-AP (EIF3D antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 10219-1-AP

    WB analysis of mouse brain using 10219-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10219-1-AP (EIF3D antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • WB analysis of L02 using 10219-1-AP

    WB analysis of L02 using 10219-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 10219-1-AP (EIF3D Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of L02 using 10219-1-AP

    WB analysis of L02 using 10219-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 10219-1-AP (EIF3D Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of L02 using 10219-1-AP

    WB analysis of L02 using 10219-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 10219-1-AP (EIF3D Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of human brain using 10219-1-AP

    WB analysis of human brain using 10219-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 10219-1-AP (EIF3D Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 10219-1-AP

    WB analysis of A549 using 10219-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 10219-1-AP (EIF3D Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 10219-1-AP

    WB analysis of HepG2 using 10219-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 10219-1-AP (EIF3D Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of A549 using 10219-1-AP

    IP experiment of A549 using 10219-1-AP

    IP result of anti-EIF3D (IP:10219-1-AP, 4ug; Detection:10219-1-AP 1:300) with A549 cells lysate 2800 ug.

  • IHC staining of human breast cancer using 10219-1-AP

    IHC staining of human breast cancer using 10219-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10219-1-AP (EIF3D Antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with EIF3D antibody included
  • IHC staining of human breast cancer using 10219-1-AP

    IHC staining of human breast cancer using 10219-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10219-1-AP (EIF3D Antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with EIF3D antibody included
  • IF Staining using 10219-1-AP

    IF Staining using 10219-1-AP

    IF result of 10219-1-AP (anti-EIF3D) in U2OS cell (treated with 100 mM sodium arsenite to cause stress-induced translational arrest) by Dr. Nancy Kedersha.U2OS cells (FAST-YFP stables; but not showing FAST-YFP); stained with PTG anti-eIF3p66 in green; and counterstained with anti-eIF3b (goat polyclonal) in red; nuclei stained blue using Hoechst.

  • IF Staining of HepG2 using 10219-1-AP

    IF Staining of HepG2 using 10219-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EIF3D antibody (10219-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • IF Staining of HepG2 using 10219-1-AP

    IF Staining of HepG2 using 10219-1-AP

    Immunofluorescent analysis of HepG2 cells using 10219-1-AP (EIF3D antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

  • IF Staining of HepG2 using 10219-1-AP

    IF Staining of HepG2 using 10219-1-AP

    Immunofluorescent analysis of Ethacrynic acid treated HepG2 cells using 10219-1-AP (EIF3D antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

  • IF Staining of HepG2 using 10219-1-AP

    IF Staining of HepG2 using 10219-1-AP

    Immunofluorescent analysis of HepG2 cells using 10219-1-AP (EIF3D antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

  • IF Staining of HepG2 using 10219-1-AP

    IF Staining of HepG2 using 10219-1-AP

    Immunofluorescent analysis of Ethacrynic acid treated HepG2 cells using 10219-1-AP (EIF3D antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

  • IF Staining of transfected cells using 10219-1-AP

    IF Staining of transfected cells using 10219-1-AP

    IF result of 10219-1-AP (anti-EIF3D) in U2OS cell by Dr. Nancy Kedersha. U2OS cells (FAST-YFP stables; but not showing FAST-YFP); stained with 10219-1-AP (EIF3D) in green; and counterstained with Santa Cruz anti-eIF3b (goat polyclonal) in red; nuclei stained blue using Hoechst.; The EIF3D(p66) appears associated with mitochondria.

  • IF Staining of MCF-7 using 10219-1-AP

    IF Staining of MCF-7 using 10219-1-AP

    Immunofluorescent analysis of MCF-7 cells, using EIF3D antibody 10219-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Tested Applications

Positive WB detected inmouse brain tissue, L02 cells, A549 cells, HepG2 cells, human brain tissue, Jurkat cells
Positive IP detected inA549 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU2OS cells, HepG2 cells, Ethacrynic acid treated HepG2 cells, transfected cells, N/A, MCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy EIF3D Ready-To-Use IHC Kit. EIF3D primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10219-1-AP targets EIF3D in WB, PLA, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, yeast samples.

Tested Reactivity human, mouse, yeast
Cited Reactivityhuman, yeast, rat, mouse, rabbit
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EIF3D fusion protein Ag0268 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 3, subunit D
Calculated molecular weight 66 kDa
Observed molecular weight 66 kDa
GenBank accession numberBC000328
Gene symbol EIF3D
Gene ID (NCBI) 8664
RRIDAB_2096880
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The mammalian translation initiation factor 3 (eIF3), is a multiprotein complex of ~600 kDa that binds to the 40 S ribosome and promotes the binding of methionyl-tRNAi and mRNA. The EIF3S7(p66) is the major RNA binding subunit in this complex. Human eIF3-p66 shares 64% sequence identity with a hypothetical Caenorhabditis elegans protein, presumably the p66 homolog. Deletion analyses of recombinant derivatives of eIF3-p66 show that the RNA-binding domain lies within an N-terminal 71-amino acid region rich in lysine and arginine.

Protocols

Product Specific Protocols
WB protocol for EIF3D antibody 10219-1-APDownload protocol
IHC protocol for EIF3D antibody 10219-1-APDownload protocol
IF protocol for EIF3D antibody 10219-1-APDownload protocol
IP protocol for EIF3D antibody 10219-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Cell

Heterogeneous Ribosomes Preferentially Translate Distinct Subpools of mRNAs Genome-wide.

Authors - Zhen Shi
View Article
humanWB

Nat Commun

A translational program that suppresses metabolism to shield the genome.

Authors - Nathan C Balukoff
View Article
humanIHC

EBioMedicine

PMID: 31669222

Authors - Hai Huang
View Article
mouseWB

Dev Cell

Controlling tissue patterning by translational regulation of signaling transcripts through the core translation factor eIF3c

Authors - Kotaro Fujii
View Article
rabbitWB

EMBO J

HCV and CSFV IRES domain II mediate eIF2 release during 80S ribosome assembly.

Authors - Locker Nicolas N
View Article
humanWB

PLoS Pathog

A Conserved Interaction between a C-Terminal Motif in Norovirus VPg and the HEAT-1 Domain of eIF4G Is Essential for Translation Initiation.

Authors - Eoin N Leen
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Tobias (Verified Customer) (10-25-2022)

clean and good antibody for wb

  • Applications: Western Blot
FH

Peter (Verified Customer) (10-24-2022)

Specific band at correct MW

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: A549
FH

Joleen (Verified Customer) (06-10-2019)

EIF3D antibody recognizes a prominent band around the correct molecular weight.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1k
  • Cell Tissue Type: MDCK GII
EIF3D Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:10219-1-AP)