Featured Product
KD/KO Validated

Ezrin Polyclonal antibody

Name: Ezrin Polyclonal antibody
Brand: Proteintech
SKU: 26056-1-AP

Ezrin Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, RIP, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 26056-1-AP

Synonyms

CVIL, CVL, Cytovillin, EZR, Ezrin, p81, VIL2, Villin 2

Validation Data Gallery

Applications:

AllWBIPIHCIF

WB analysis using 26056-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 26056-1-AP (Ezrin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 26056-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 26056-1-AP (Ezrin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 26056-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 26056-1-AP (Ezrin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 26056-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 26056-1-AP (Ezrin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 26056-1-AP
IP result of anti-Ezrin (IP:26056-1-AP, 4ug; Detection:26056-1-AP 1:2000) with HeLa cells lysate 3200ug.
IHC staining of mouse stomach using 26056-1-AP
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 26056-1-AP (Ezrin antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with Ezrin antibody included
IHC staining of mouse stomach using 26056-1-AP
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 26056-1-AP (Ezrin antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with Ezrin antibody included
IHC staining of mouse stomach using 26056-1-AP
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 26056-1-AP (Ezrin antibody) at dilution of 1:8000 (under 40x lens).
Ready-to-use IHC kits available with Ezrin antibody included
IHC staining of human small intestine using 26056-1-AP
Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 26056-1-AP (Ezrin antibody) at dilution of 1:2000 (under 10x lens).
Ready-to-use IHC kits available with Ezrin antibody included
IHC staining of human small intestine using 26056-1-AP
Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 26056-1-AP (Ezrin antibody) at dilution of 1:2000 (under 40x lens).
Ready-to-use IHC kits available with Ezrin antibody included
IF Staining of HeLa using 26056-1-AP
Immunofluorescent analysis of HeLa cells using 26056-1-AP (Ezrin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 26056-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, mouse kidney tissue, NIH/3T3 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	mouse stomach tissue, human small intestine tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	HeLa cells

Planning an IHC experiment? We recommend our IHCeasy Ezrin Ready-To-Use IHC Kit. Ezrin primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)	IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

KD/KO	See 3 publications below
WB	See 11 publications below
IHC	See 1 publications below
IF	See 2 publications below
RIP	See 1 publications below

Product Information

26056-1-AP targets Ezrin in WB, RIP, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Ezrin fusion protein Ag23530 相同性解析による交差性が予測される生物種
Full Name	ezrin
Calculated molecular weight	69 kDa
Observed molecular weight	70-75 kDa
GenBank accession number	BC013903
Gene symbol	EZR
Gene ID (NCBI)	7430
RRID	AB_2722561
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for Ezrin antibody 26056-1-AP	Download protocol
IHC protocol for Ezrin antibody 26056-1-AP	Download protocol
IF protocol for Ezrin antibody 26056-1-AP	Download protocol
IP protocol for Ezrin antibody 26056-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human,mouse	IF	Acta Neuropathol Selective vulnerability of tripartite synapses in amyotrophic lateral sclerosis. Authors - Matthew J Broadhead View Article
human	WB	Proc Natl Acad Sci U S A Cotranslational interaction of human EBP50 and ezrin overcomes masked binding site during complex assembly. Authors - Krishnendu Khan KO Validated View Article
mouse	WB	Elife Gq activity- and β-arrestin-1 scaffolding-mediated ADGRG2/CFTR coupling are required for male fertility. Authors - Dao-Lai Zhang View Article
human	WB	Int J Mol Sci Bidirectional Tumor-Promoting Activities of Macrophage Ezrin. Authors - Krishnendu Khan KD Validated View Article
mouse	WB	J Biol Chem Ciliary neurotrophic factor (CNTF) protects retinal cone and rod photoreceptors by suppressing excessive formation of the visual pigments. Authors - Songhua Li View Article
human	WB	Sci Rep Quantitative Proteomic analysis on Activated Hepatic Stellate Cells reversion Reveal STAT1 as a key regulator between Liver Fibrosis and recovery. Authors - Hongyu Zhang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Ezrin Polyclonal antibody

Ezrin Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Validation Data Gallery

WB analysis using 26056-1-AP

WB analysis of HeLa using 26056-1-AP

WB analysis of mouse kidney using 26056-1-AP

WB analysis of NIH/3T3 using 26056-1-AP

IP experiment of HeLa using 26056-1-AP

IHC staining of mouse stomach using 26056-1-AP

IHC staining of mouse stomach using 26056-1-AP

IHC staining of mouse stomach using 26056-1-AP

IHC staining of human small intestine using 26056-1-AP

IHC staining of human small intestine using 26056-1-AP

IF Staining of HeLa using 26056-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Selective vulnerability of tripartite synapses in amyotrophic lateral sclerosis.

Cotranslational interaction of human EBP50 and ezrin overcomes masked binding site during complex assembly.

Gq activity- and β-arrestin-1 scaffolding-mediated ADGRG2/CFTR coupling are required for male fertility.

Bidirectional Tumor-Promoting Activities of Macrophage Ezrin.

Ciliary neurotrophic factor (CNTF) protects retinal cone and rod photoreceptors by suppressing excessive formation of the visual pigments.

Quantitative Proteomic analysis on Activated Hepatic Stellate Cells reversion Reveal STAT1 as a key regulator between Liver Fibrosis and recovery.