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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LARP1 Monoclonal antibody

LARP1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2E9F2

Cat no : 67810-1-Ig

Synonyms

KIAA0731, La related protein 1, LARP, LARP1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LARP1 Monoclonal antibody (67810-1-Ig)

    WB analysis using 67810-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67810-1-Ig (LARP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using LARP1 Monoclonal antibody (67810-1-Ig)

    WB analysis of HSC-T6 using 67810-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67810-1-Ig (LARP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using LARP1 Monoclonal antibody (67810-1-Ig)

    WB analysis of NIH/3T3 using 67810-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67810-1-Ig (LARP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using LARP1 Monoclonal antibody (67810-1-Ig)

    IHC staining of human ovary tumor using 67810-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67810-1-Ig (LARP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using LARP1 Monoclonal antibody (67810-1-Ig)

    IHC staining of human ovary tumor using 67810-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67810-1-Ig (LARP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LARP1 Monoclonal antibody (67810-1-Ig)

    IF Staining of HepG2 using 67810-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using LARP1 antibody (67810-1-Ig, Clone: 2E9F2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). Blue (DAPI).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LARP1 Monoclonal antibody (67810-1-Ig)

    IF Staining of HepG2 using 67810-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using LARP1 antibody (67810-1-Ig, Clone: 2E9F2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inHCT 116 cells, HSC-T6 cells, NIH/3T3 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67810-1-Ig targets LARP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen LARP1 fusion protein Ag29993 相同性解析による交差性が予測される生物種
Full Name La ribonucleoprotein domain family, member 1
Calculated molecular weight 1096 aa, 124 kDa
Observed molecular weight140-150 kDa
GenBank accession numberBC033856
Gene symbol LARP1
Gene ID (NCBI) 23367
RRIDAB_2918573
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for LARP1 antibody 67810-1-IgDownload protocol
IHC protocol for LARP1 antibody 67810-1-IgDownload protocol
IF protocol for LARP1 antibody 67810-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols