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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

P53 Monoclonal antibody

P53 Monoclonal Antibody for ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2D2F10

Cat no : 60283-1-Ig

Synonyms

Antigen NY CO 13, Cellular tumor antigen p53, LFS1, Phosphoprotein p53, TRP53, tumor protein p53, Tumor suppressor p53

Validation Data Gallery

Applications:
  • WB analysis of NIH/3T3 using 60283-1-Ig

    WB analysis of NIH/3T3 using 60283-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60283-1-Ig (P53 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of RAW 264.7 using 60283-1-Ig

    WB analysis of RAW 264.7 using 60283-1-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60283-1-Ig (P53 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of A431 using 60283-1-Ig

    WB analysis of A431 using 60283-1-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 60283-1-Ig (P53 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 60283-1-Ig

    WB analysis of HEK-293 using 60283-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 60283-1-Ig (P53 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 60283-1-Ig

    WB analysis of HEK-293 using 60283-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 60283-1-Ig (P53 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of COLO 320 using 60283-1-Ig

    WB analysis of COLO 320 using 60283-1-Ig

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 60283-1-Ig (P53 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 60283-1-Ig

    IHC staining of human ovary tumor using 60283-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60283-1-Ig (P53 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary tumor using 60283-1-Ig

    IHC staining of human ovary tumor using 60283-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60283-1-Ig (P53 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

60283-1-Ig targets P53 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen P53 fusion protein Ag0698 相同性解析による交差性が予測される生物種
Full Name tumor protein p53
Calculated molecular weight 44 kDa
GenBank accession numberBC003596
Gene symbol TP53
Gene ID (NCBI) 7157
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TP53, also named as P53 and NY-CO-13, belongs to the p53 family. It has 9 isoforms. In SDS-Page, the MW is about 53kd. TP53 acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. It is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. The antibody reacts specifically with 53 kDa protein.

Protocols

Product Specific Protocols
WB protocol for P53 antibody 60283-1-IgDownload protocol
IHC protocol for P53 antibody 60283-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Ther Nucleic Acids

miR-552 Regulates Liver Tumor-Initiating Cell Expansion and Sorafenib Resistance.

Authors - Tao Han
View Article
humanWB

Oncogenesis

MeCP2 facilitates breast cancer growth via promoting ubiquitination-mediated P53 degradation by inhibiting RPL5/RPL11 transcription.

Authors - DongDong Tong
View Article
humanWB,IHC

Cancer Lett

TAZ regulates cell proliferation and sensitivity to vitamin D3 in intrahepatic cholangiocarcinoma.

Authors - Heng Xiao
  • KD Validated
View Article
human,mouseWB

Am J Cancer Res

Asparaginyl endopeptidase (AEP) regulates myocardial apoptosis in response to radiation exposure via alterations in NRF2 activation.

Authors - Lu Cao
View Article
humanWB

Am J Cancer Res

Loss of BRMS2 induces cell growth inhibition and translation capacity reduction in colorectal cancer cells.

Authors - Yaofu Liu
View Article
humanWB

Biochem Pharmacol

Ketoplatin in triple-negative breast cancer cells MDA-MB-231: High efficacy and low toxicity, and positive impact on inflammatory microenvironment.

Authors - Zhong-Ying Ma
View Article